N AND R CADHERINS IN THE DEVELOPING TELEOST RETINA

发育中的 TELEOST 视网膜中的 N 和 R 钙粘蛋白

• 批准号: 2882888
• 负责人: QIN LIU
• 金额: $ 4万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-03-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2882888
• 关键词: alternatives to animals in research; biological signal transduction; cadherins; cell cell interaction; cell differentiation; chimeric proteins; developmental neurobiology; electron microscopy; gene expression; genetically modified animals; goldfish; histogenesis; organ culture; protein structure function; retina; zebrafish

Developmental biologists have long been trying to determine mechanisms underlying the development of vertebrate retina. One of the most fundamental questions to be asked is what are the factors determining or influencing the fate of progenitor cells (e.g. what determines whether the cells become cones instead of rods). Cadherins, in addition to being responsible for adhesion between many types of cells, may also play key roles in cell differentiation, axon pathfinding and synapse formation. Our preliminary results show that both N- and R-cadherins are located in the teleost retina at a time when retinal cells are proliferating and differentiating, and when retinal ganglion cell axons are migrating out of the retina toward their major target in the brain, the optic tectum. The proposed study will first examine the developmental profiles of these two cadherins in the visual system to look for correlations between the distribution of these molecules and the major morphogenetic events of the system. Functional analysis using either cadherin antibodies in an organ culture system or injection of antisense nucleotides to block cadherin mRNA will be employed to assess the role(s) of these cadherin molecules in the development of teleost visual structures.

发育生物学家长期以来一直试图确定机制 脊椎动物视网膜发育的基础。 最有之一 要问的基本问题是决定因素是什么 或影响祖细胞的命运（例如，什么决定了 细胞是否变成锥体而不是杆体）。 此外，钙粘蛋白 负责多种类型细胞之间的粘附，也可能 在细胞分化、轴突寻路和突触中发挥关键作用 形成。 我们的初步结果表明 N- 和 R-钙粘蛋白 当视网膜细胞处于 增殖和分化，当视网膜神经节细胞轴突 正在从视网膜迁移到大脑中的主要目标， 视顶盖。 拟议的研究将首先检查 这两种钙粘蛋白在视觉系统中的发育概况 寻找这些分子的分布之间的相关性 系统的主要形态发生事件。 泛函分析使用 器官培养系统中的钙粘蛋白抗体或注射 阻断钙粘蛋白 mRNA 的反义核苷酸将用于评估 这些钙粘蛋白分子在硬骨鱼发育中的作用 视觉结构。