NONDIAMAGNETIC AGENTS IN IN VIVO 23 NA AND 1H20 MR

非磁性剂体内 23 NA 和 1H20 MR

• 批准号: 2756759
• 负责人: CHARLES S. SPRINGER
• 金额: $ 21.23万
• 依托单位: BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-04-01 至 2002-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2756759
• 关键词: baboons; bioengineering /biomedical engineering; biomagnetism; biophysics; blood brain barrier; cell morphology; contrast media; dogs; hydrogen ions; image processing; intravenous administration; laboratory rat; magnetic resonance imaging; neuroanatomy; nuclear magnetic resonance spectroscopy; sodium ion; surgery; water; baboons; bioengineering /biomedical engineering; biomagnetism; biophysics; blood brain barrier; cell morphology; contrast media; dogs; hydrogen ions; image processing; intravenous administration; laboratory rat; magnetic resonance imaging; neuroanatomy; nuclear magnetic resonance spectroscopy; sodium ion; surgery; water

The work proposed will continue to explore and demonstrate the range of applications of the effects of non-diamagnetic agents on the two strongest tissue NMR signals: those of 1H2O and 23Na-aq. The adjective non-diamagnetic is used here to indicate any magnetic behavior (ex. paramagnetic, super-paramagnetic, ferromagnetic, etc.) exhibited in addition to simple diamagnetism. Specific projects in the first general category (1H2O) are proposed. The genesis of some of these ideas derived from early work with 23Na-aq. However, now priority must be given to the former, over analogous studies of the latter. It will be demonstrated that one can make 1H2O MR images of contrast reagent (CR) distribution volumes directly, as well as cytolemmal water permeability coefficient maps, cytoplasmic; volume images, and extracellular volume maps. The latter two represent the editing of quantitative (spin density) 1H2O images for the fundamental compartmentalization of biology, which can be radically altered in pathologies such as edemas and tumors. The advance making these development possible is the new technique of combined relaxography and imaging (CRI) recently introduced by this laboratory. The CRI technique is explained in this proposal, and is totally general and applicable to longitudinal, transverse, or rotating-frame NMR relaxation. It can be used to study any CR, whether employing the hyperfine or the bulk magnetic susceptibility mechanisms (or both). Longitudinal relaxation and hyperfine CRs are emphasized in this proposal. This program has recently moved to a new institution and a significantly higher level of effort. Thus, in addition to rat experiments, studies of canine (beagle) and primate (baboon) models are proposed. These involve the programmed, stepped IV infusions of CRs approved for humans, and a quite precedented levels. The ability to study the larger animals provides the opportunity to address important issues of the scaling of the pharmacokinetics and attainable image resolution. The types of images itemized above will be examined for any tissue present in any selected field-of-view, including various muscle groups, the liver, a tumor model implanted in rat thigh muscle, the brain, and a tumor model implanted in the rat brain. The latter two require direct intracerebroventricular injection of the CR since it does not cross the blood-brain-barrier in normal tissue and this approach will be applied only to rats and dogs. The tumor models allow assessment of much greater tissue heterogeneity, and the fundamental bases of their study by the increasingly popular dynamic CR-enhanced method, which involves bolus IV injections. This work involves aspects of physics, physical chemistry, biophysics, and physiology and has ramifications in the study of a number of pathological conditions including neurological and cardiovascular disorders.

提议的工作将继续探索并证明 非二号磁剂对两者的影响的应用 最强的组织NMR信号：1H2O和23NA-AQ的组织信号。形容词 这里使用非磁磁性指示任何磁性行为（ex。 顺磁性，超顺磁性，铁磁等） 除简单的磁磁性。 第一个将军中的特定项目 提出了类别（1H2O）。 其中一些想法的起源 源自23na-aq的早期工作。 但是，现在的优先级必须是 给出了前者的类似研究。 可以证明可以制作1H2O MR的对比图像 试剂（CR）分布量直接以及细胞膜水 渗透系数图，细胞质；音量图像，和 细胞外体积图。 后两个代表编辑 基本的定量（自旋密度）1H2O图像 生物学的分室化，可以从根本上改变 诸如Edemas和肿瘤等病理。 提前使这些 可以开发的是宽松和合并的新技术 该实验室最近引入的成像（CRI）。 CRI技术 在此提案中解释了，并且完全适用于 纵向，横向或旋转框架NMR松弛。 可以 用于研究任何CR，无论是使用超细 磁化性机制（或两者都）。 纵向松弛 在该提案中强调了Hyperfine CRS。这个程序有 最近搬到了一个新机构，并明显更高的水平 努力。 因此，除了大鼠实验外，犬的研究 提出了（小猎犬）和灵长类动物（狒狒）模型。 这些涉及 已编程，逐步批准了CRS的IV IV输注， 先例水平。 研究大动物的能力提供 解决缩放的重要问题的机会 药代动力学和可实现的图像分辨率。 图像的类型 上面逐项列出的将在任何选定中存在的任何组织检查 视野视野，包括各种肌肉组，肝脏，肿瘤模型 植入大鼠大腿肌肉，大脑和植入肿瘤模型 在大鼠大脑中。 后两个需要直接脑室脑室 注射CR，因为它不会越过血脑屏障 正常组织和这种方法仅适用于大鼠和狗。 肿瘤模型允许评估更大的组织异质性， 以及越来越受欢迎的研究的基本基础 动态CR增强方法，涉及注射推注。 这 工作涉及物理，物理化学，生物物理学和 生理学，并在许多研究中产生了影响 病理状况，包括神经和心血管 疾病。