NEAR FIELD OPTICAL POINT MEASUREMENTS ON LIVING CELLS

活细胞近场光点测量

• 批准号: 2714282
• 负责人: Michael J Szulczewski
• 金额: $ 35.5万
• 依托单位: PRAIRIE TECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-01 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2714282
• 关键词: biomedical equipment development; method development; microscopy; optics

A prototype biological near-field microscope is currently in use at the Iowa State University where experiments in a new world of sub- diffraction limited optical measurements of molecular interaction in living cells are now taking place. Areas of improvement to this prototype have been identified to produce a commercial product. The goal of this phase II proposal is to develop and commercialize a near- field microscope for the biological research market. Development of a X, Y, Z near-field positioning device which integrates stepper motors and piezo devices for course and fine movements respectively will meet the positioning requirements for near-field microscopy. Integrated software utilizing object oriented software programs will provide a visual, mouse driven point and click control screen. Incorporation of these improvements will result in a highly reliable turn-key biological near-field microscope. PROPOSED COMMERCIAL APPLICATION: Completion of phase II will produce a commercial near-field microscope product designed for the biological research market. This product will create a multi-million dollar niche market. Applications will be abundant. The near-field microscope is the tool that will allow new discoveries in many new areas of investigations such as monitoring molecular interactions in living cells. Continued development of photon density feedback will provide additional commercial applications: 1) molecular studies of thin films 2) automated electrophysiology probe placement 3) uv photolysis and 4) atomic force microscopy.

目前正在使用原型生物近场显微镜 爱荷华州立大学，在一个新世界的实验 衍射有限的分子相互作用的光学测量 现在正在发生活细胞。 改进领域 已经确定了原型生产商业产品。 这 该第二阶段提案的目标是开发和商业化近乎 - 生物研究市场的现场显微镜。 开发X，Y，Z近场定位设备，该设备集成 步进电动机和压电设备，用于课程和精美动作 分别将满足近场的定位要求 显微镜。 利用面向对象软件的集成软件 程序将提供视觉，鼠标驱动点，并单击控制 屏幕。 结合这些改进将导致高度 可靠的交钥匙生物学近场显微镜。 拟议的商业应用： II期的完成将产生商业近场显微镜 为生物学研究市场设计的产品。该产品将 创建一个数百万美元的利基市场。申请将是 丰富。 近场显微镜是将允许新的工具 在许多新调查领域的发现，例如监视 活细胞中的分子相互作用。 光子的持续发展 密度反馈将提供其他商业应用：1） 薄膜的分子研究2）自动电生理探针 位置3）紫外光解和4）原子力显微镜。