MECHANISM AND REGULATION OF SFT FUNCTION IN FE TRANSPORT

FE传输中SFT功能的机制和调节

• 批准号: 2776011
• 负责人: Marianne Wessling-Resnick
• 金额: $ 5.86万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-04-15 至 1999-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2776011
• 关键词: SDS polyacrylamide gel electrophoresis; Xenopus oocyte; alkaline phosphatase; cell membrane; clone cells; gel mobility shift assay; gene expression; genetic regulatory element; genetic translation; immunofluorescence technique; intracellular membranes; ion transport; iron; membrane transport proteins; microinjections; molecular cloning; northern blottings; nucleic acid sequence; polymerase chain reaction; protein biosynthesis; protein structure function; regulatory gene; site directed mutagenesis; transferrin; yeast two hybrid system

DESCRIPTION: Dr. Wessling-Resnick's overall aim is to understand the mechanism of iron transport across membranes. This is a very important problem both from the clinical and basic biology point of view. She has identified a protein called SFT, which is required for the Transferrin independent transport of Fe in Xenopus oocytes and in a preerythroid cell-line K562. SFT has some similarities with the ABC or ATP-Binding Cassette transporters. The three major aims of this proposal are: (1) to characterize the role of SFT in Fe transport; (2) to understand how SFT is localized to both the plasma membrane and endosomes; and (3) to identify the cis-acting elements that regulate the intracellular levels of SFT.

描述：Wessling-Resnick博士的总体目的是了解 铁跨膜的机理。 这是一个非常重要的 从临床和基本生物学的角度来看的问题。 她有 鉴定出一种称为SFT的蛋白质，这是转铁蛋白所必需的 在异爪蟾卵母细胞和荷花前的Fe独立运输 细胞线K562。 SFT与ABC或ATP结合有一些相似之处 盒式转运蛋白。 该提案的三个主要目的是：（1） 表征SFT在Fe运输中的作用； （2）了解SFT的样子 位于质膜和内体上； （3）确定 调节细胞内SFT水平的顺式作用元件。