CYTOCHROME P450 AND LUNG IN HUMAN PRETERM INFANTS

人类早产儿的细胞色素 P450 和肺

• 批准号: 2796817
• 负责人: ROBERT B COTTON
• 金额: $ 24.76万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-09-30 至 2001-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2796817
• 关键词: arachidonate; bronchopulmonary dysplasia; chemoprevention; cimetidine; clinical research; clinical trials; cytochrome P450; disease /disorder proneness /risk; electrospray ionization mass spectrometry; enzyme activity; enzyme linked immunosorbent assay; free radical oxygen; gas chromatography; human subject; human therapy evaluation; isotope dilution method; lung injury; lung lavage; peroxidation; premature infant human; respiratory disorder chemotherapy; respiratory gas transport; urinalysis

DESCRIPTION (Adapted from the Investigator's Abstract): The goal of this project is to determine the role of lung cyt P-450 in mediating lung injury in the newborn premature infant at risk for BPD. Aim 1 is to determine if the cyt P-450 inhibitor cimetidine will reduce early lung injury, as reflected by pulmonary gas exchange, in human premature infants at risk for BPD. Aim 2 is to determine the degree of lipid peroxidation in study patients by measuring F2-isoprostane levels in tracheobronchial aspirates and in urine. Aim 3 is to identify P-450 mediated arachidonic acid (AA) metabolites in tracheobronchial aspirates obtained from patients enrolled in the study and to correlate the levels of specific metabolites with the assigned treatment and gas exchange index. Aim 4 is to identify inflammatory markers in tracheobronchial aspirates and urine obtained from study patients in order to determine whether the anticipated reduction in O2 free radical generation and P-450 AA metabolite production in patients receiving cimetidine is also accompanied by a reduced inflammatory response.

描述（改编自研究者的摘要）：本研究的目标 该项目旨在确定肺细胞色素 P-450 在介导肺损伤中的作用 有 BPD 风险的新生儿早产儿。 目标 1 是确定是否 cyt P-450 抑制剂西咪替丁可减少早期肺损伤，因为 通过肺部气体交换反映，人类早产儿有以下风险： 边缘性人格障碍。 目标 2 是确定研究中脂质过氧化的程度 通过测量气管支气管抽吸物中的 F2-异前列烷水平来评估患者 和尿液中。 目标 3 是鉴定 P-450 介导的花生四烯酸 (AA) 从入组患者获得的气管支气管抽吸物中的代谢物 该研究并将特定代谢物的水平与 指定的处理和气体交换指数。 目标 4 是确定 气管支气管抽吸物和尿液中的炎症标志物 研究患者以确定预期的 O2 减少是否 患者体内自由基的产生和 P-450 AA 代谢物的产生 接受西咪替丁也伴随着炎症反应的减轻。