REGULATION OF PROLACTIN SECRETION AT THE LACTOTROPH

泌乳素泌乳素分泌的调节

• 批准号: 2701092
• 负责人: MARC Edward FREEMAN
• 金额: $ 21.74万
• 依托单位: FLORIDA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-05-01 至 2000-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2701092
• 关键词: G protein; antisense nucleic acid; arachidonate; biological signal transduction; calcium; calcium channel; dopamine; dopamine receptor; eicosanoid metabolism; electrophysiology; fluorescence microscopy; hormone regulation /control mechanism; hypothalamic pituitary axis; laboratory rat; neurohormones; phospholipase A2; pituitary gland; prolactin; prolactin releasing /inhibiting factor; receptor coupling; secretion; tissue /cell culture; voltage /patch clamp

Prolactin (pRL) is a hormone which originates in cells of the anterior pituitary gland, lactotrophs, and plays multiple physiological roles in all mammals. Not only does it promote lactation in all mammals, but it also plays a role in maternal behavior, maintenance of pregnancy as well as osmoregulation and the immune response. Dopamine (DA) is a well established physiological inhibitor of PRL secretion. It originates in cells, neurons, within a specialized area of the brain, the hypothalamus, which sends projections that terminate upon the vasculature connecting the hypothalamus with the pituitary gland. It has recently been shown that DA is also a physiological stimulator of PRL secretion. The long term objective of this application is to characterize the mechanism whereby DA has opposing physiological effects on PRL secretion. Using measurements of PRL secretion as well as intracellular transduction events, the Specific Aims of this project will be to: (1) determine which DA receptors on the lactotroph are responsible for stimulation of PRL secretion, (2) determine which G-proteins are linked to stimulation versus inhibition of PRL secretion by DA, (3) determine if the intracellular lipids are involved in the stimulation of PRL secretion by DA and (4) determine the role of calcium in DA-stimulated PRL secretion. Since PRL has so many multi- faceted roles in the physiology of the organism, it is critical for purposes of possible therapeutic approaches to diseases involving PRL that all aspects of its physiological control be clarified. For example, both benign and malignant diseases of the mammary gland are approached by manipulating pituitary PRL secretion. Thorough knowledge of the control of PRL secretion will allow other approaches to its manipulation in disease states.

催乳素（PRL）是一种激素，起源于前室的细胞 垂体，乳酸营养，并在 所有哺乳动物。它不仅促进了所有哺乳动物的泌乳，而且还可以 还在孕产妇行为中发挥作用，维持怀孕 作为渗透调节和免疫反应。多巴胺（DA）是一口井 建立的PRL分泌的生理抑制剂。它起源于 细胞，神经元，在大脑的专业区域内，下丘脑， 发送的投影终止于连接的脉管系统 下丘脑与垂体。最近已经证明了DA 也是PRL分泌的生理刺激剂。长期 该应用的目的是表征DA的机制 对PRL分泌具有相反的生理影响。使用的测量 PRL分泌以及细胞内转导事件，特定 该项目的目的是：（1）确定哪些DA受体 乳营养素负责刺激PRL分泌，（2）确定 哪种G蛋白与PRL的刺激与抑制有关 DA的分泌，（3）确定细胞内脂质是否参与 DA和（4）对PRL分泌的刺激确定 DA刺激的PRL分泌中的钙。由于PRL有很多多 在生物体的生理学中角色，对 涉及PRL的疾病的可能治疗方法的目的 澄清其生理控制的所有方面。例如，两者 乳腺的良性和恶性疾病已通过 操纵垂体PRL分泌。对控制的彻底了解 PRL分泌将允许其他方法来操纵疾病 国家。