ANESTHETIC ACTIONS ON INHIBITORY GABAERGIC INTERNEURONS

对抑制性伽巴能中间神经元的麻醉作用

• 批准号: 2750163
• 负责人: M. Bruce MacIver
• 金额: $ 19.16万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-08-01 至 2000-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2750163
• 关键词: GABA receptor; action potentials; general anesthesia; interneurons; pharmacokinetics; voltage /patch clamp

DESCRIPTION: (Adapted from the applicant's abstract) The goal of the investigators is to understand mechanisms of general anesthetic action. Attenuated neuronal excitability during anesthesia results in part from enhanced GABA-mediated synaptic inhibition. The major direction of this proposal is to investigate anesthetic actions on inhibitory GABAergic interneurons. Interneurons mediate synaptic inhibition by releasing GABA. Whole-cell recordings and infrared imaging make it now possible to study anesthetic actions on previous difficult to access interneurons. Unexpectedly, because general anesthetics usually depress transmitter release, is the preliminary finding that general anesthetics increase the spontaneous release of GABA from inhibitory interneurons. Enhanced GABA release can combine with anesthetic-postsynaptic GABA receptor actions to augment inhibition. The proposed study will identify the effects of anesthetics on inhibitory GABAergic interneurons, characterize the mechanisms underlying these actions, and determine the consequences of these effects on neuronal excitability. The investigators believe that these studies on inhibitory GABAergic interneurons will provide information regarding mechanisms of anesthetic action.

描述：（根据申请人的摘要进行了改编） 研究人员将了解一般麻醉作用的机制。 麻醉期间的神经元兴奋性减弱。 增强的GABA介导的突触抑制作用。 这是主要方向 建议是研究对抑制性GABA能的麻醉作用 中间神经元。 中神经元通过释放GABA介导突触抑制。 全细胞录制和红外成像使现在可以学习 对以前难以访问中间神经元的麻醉作用。 出乎意料的是，因为全身麻醉通常会降低发射器 释放是总体麻醉剂增加的初步发现 从抑制性神经元中自发释放GABA。 增强的GABA 释放可以与麻醉症的加巴受体作用结合起来 增强抑制作用。 拟议的研究将确定 关于抑制性GABA能中间神经元的麻醉剂，表征 这些行动的基础机制，并确定这些行动的后果 对神经元兴奋性的影响。 调查人员认为这些 抑制性GABA能中间神经元的研究将提供信息 关于麻醉作用的机制。