TRANSCRIPTIONAL CONTROL OF OSTEOBLAST STRESS RESPONSES

成骨细胞应激反应的转录控制

• 批准号: 2770251
• 负责人: LYNDON F COOPER
• 金额: $ 8.13万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-09-01 至 2001-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2770251
• 关键词: actins; endosseous dental implant; estrogen receptors; gene induction /repression; genetic transcription; genetically modified animals; laboratory mouse; osteoblasts; recombinant proteins; stress proteins; actins; endosseous dental implant; estrogen receptors; gene induction /repression; genetic transcription; genetically modified animals; laboratory mouse; osteoblasts; recombinant proteins; stress proteins

DESCRIPTION: The goals of this career development plan are to contribute to osteoblast biology and endosseous implant related activities through fundamental studies of gene transcription, to establish a laboratory that will provide exemplary training of students, fellows, and clinician-scientists, and to develop clinical research interests supported by and complimentary to these research interests. This will be achieved through a) one year of formal training in a combination of three leading molecular biology laboratories at UNC, and b) four years of 75% research effort devoted towards accomplishing the specific aims of the research project. The aims are to investigate: 1) transcriptional suppression as a mechanism for tissue-specific constitutive expression of hsp27; 2) estrogenic control of hsp27 transcription in osteoblasts; and 3) the chaperone function of hsp27 in osteoblasts. Dr. Roland Arnold, Director of the Dental Research Center, will serve as lead sponsor of this career development program. Dr. Cooper plans to attend two relevant basic research meetings/symposia per year, while other travel to dental research meetings will be covered by the applicant s R29 award and departmental funds. Carolina Workshops on transgenic mice, on construction and screening of peptide phage display libraries, and on biomedical microscopy are planned and budgeted for the first three years of the award period.

描述：该职业发展计划的目标是为 成骨细胞生物学和室内植入物与相关的活动 基因转录的基本研究，以建立一个实验室 将提供对学生，研究员和 临床医生科学家，并发展支持的临床研究兴趣 通过并免费获得这些研究兴趣。 这将被实现 通过a）三个领先的一年正规培训 UNC的分子生物学实验室，b）75％研究的四年 致力于完成研究的具体目标 项目。 目的是调查：1）转录抑制作 HSP27组织特异性本构表达的机制； 2） 成骨细胞中Hsp27转录的雌激素控制； 3） HSP27在成骨细胞中的伴侣函数。 罗兰·阿诺德（Roland Arnold）博士 牙科研究中心将担任此职业的首席赞助商 发展计划。 库珀博士计划参加两项相关的基础研究 每年会议/研讨会，而其他前往牙科研究会议 申请人S R29奖和部门资金将涵盖。 关于转基因小鼠的卡罗来纳州讲习班，建造和筛查 计划肽噬菌体展示库和生物医学显微镜检查 并在奖励期的头三年预算。