NUCLEAR IMPORT OF PREMRNA BINDING PROTEINS

前RNA结合蛋白的核导入

• 批准号: 2750068
• 负责人: SERAFIN PINOL-ROMA
• 金额: $ 22.38万
• 依托单位: ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-08-01 至 2001-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2750068
• 关键词: DNA directed RNA polymerase; RNA binding protein; Xenopus oocyte; antibody; genetic transcription; heterogeneous nuclear ribonucleoprotein; human immunodeficiency virus; intracellular transport; laboratory mouse; microinjections; molecular cloning; posttranslational modifications; precursor mRNA; tissue /cell culture; virus protein

DESCRIPTION: Several hnRNP proteins cycle between the nucleus and cytoplasm, exiting the nucleus associated with mRNA and re-entering by a unique mechanism that is dependent on continued transcription by RNA polymerase II. This suggests that regulatory signals are transduced between the nucleus and cytoplasm. Preliminary fractionation of cell extracts used to support protein import in permeabilized cells indicates that the factors necessary for the import of classical NLS-containing proteins can be separated from those required for hnRNP A1 protein. This proposal aims to purify and characterize the factors required for nuclear import of hnRNP A1. Their regulation by RNA polymerase II transcription will be addressed. Both permeabilized cells and microinjection into Xenopus oocytes will be used to identify relevant factors.

描述：细胞核之间的几种HNRNP蛋白周期 细胞质，退出与mRNA相关的细胞核，并通过 取决于RNA持续转录的独特机制 聚合酶II。 这表明调节信号在 细胞核和细胞质。 细胞提取物的初步分馏 支持在透化细胞中进口的蛋白质表明这些因素 进口含NLS的蛋白质所需的必要 与HNRNP A1蛋白所需的分离。 该建议旨在 纯化并表征HNRNP A1核进口所需的因素。 将解决它们对RNA聚合酶II转录的调节。 两个都 透化细胞和对爪蟾卵母细胞的微注射将用于 确定相关因素。