SURFACE CONFINED COLLOIDAL AU--PROTEIN COMPLEXES

表面限制胶体AU--蛋白质复合物

• 批准号: 2634833
• 负责人: MICHAEL J. NATAN
• 金额: $ 10.59万
• 依托单位: PENNSYLVANIA STATE UNIVERSITY, THE
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-01-01 至 1999-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2634833
• 关键词: Raman spectrometry; colloids; electrochemistry; enzymes; gold; immunoglobulins; metal complex

DESCRIPTION: The long-term objective of this research is to develop a new, general paradigm for construction of biosensors based on complexes between proteins and colloidal Au nanoparticles. This idea is founded on the hypothesis that appropriately-sized colloidal Au nanoparticles can be used as non-denaturing photon and electron antennae for proteins, allowing protein:Au colloid complexes to be used as building blocks for a variety of sensing mechanisms. To that end, the proposed work will demonstrate direct electrochemistry of enzyme:Au complexes. It will also show that antibody:Au complexes can be used to detect small-molecule antigens by surface enhanced Raman scattering (SERS), to improve the mass resolution in surface plasmon resonance spectroscopy, and to detect protein antigens by binding-induced changes in electrical resistance. Preliminary studies have already shown that: (i) reversible electrochemistry of cytochrome c is obtained at Au colloid-modified electrodes without any pretreatment; (ii) covalent attachment of Cc:Au complexes to roughened Ag surfaces facilitates acquisition of SERS spectra; (iii) electrically conductive surfaces can be prepared by Au colloid multilayer assembly; and (iv) Au particle size impacts SERS enhancement factors, electrochemical reversibility, and enzymatic activity. These data show that colloidal AU particles are unique in their ability to interact favorably with photons, electrons, and proteins, as well as to be assembled into well-defined, easily-characterized macroscopic surfaces.

描述：这项研究的长期目标是开发一个新的， 基于复合物之间建造生物传感器的一般范式 蛋白质和胶体Au纳米颗粒。 这个想法建立在 可以使用适当大小的胶体Au纳米颗粒的假设 作为蛋白质的非换性光子和电子触角，允许 蛋白质：Au胶体复合物，用作多种 感应机制。 为此，拟议的工作将直接证明 酶的电化学：AU综合体。 它还将表明抗体：au 复合物可用于通过表面增强的小分子抗原检测小分子抗原 拉曼散射（SERS），以改善表面等离子体的质量分辨率 共振光谱和通过结合诱导的蛋白质抗原检测 电阻的变化。 初步研究已经表明：（i）可逆 在Au胶体修饰处获得细胞色素C的电化学 电极没有任何预处理； （ii）CC的共价附件：AU 粗糙的Ag表面的复合物有助于获得SERS光谱； （iii）可以通过AU胶体制备导电表面 多层组件； （iv）AU粒径会影响SERS增强 因素，电化学可逆性和酶活性。 这些数据 表明胶体Au颗粒的相互作用能力是独一无二的 具有光子，电子和蛋白质以及组装有利 进入定义明确，易于特征的宏观表面。