REGULATION OF CHOLINERGIC FUNCTION IN C ELEGANS

线虫胆碱能功能的调节

• 批准号: 2711304
• 负责人: DENNIS L FRISBY
• 金额: $ 3.15万
• 依托单位: OKLAHOMA MEDICAL RESEARCH FOUNDATION
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-12-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2711304
• 关键词: Caenorhabditis elegans; RNA splicing; acetylcholine; alternatives to animals in research; animal genetic material tag; choline acetyltransferase; cholinergic receptors; developmental genetics; enzyme activity; gene induction /repression; genetic promoter element; genetically modified animals; membrane transport proteins; neurogenetics; neurons; neurotransmitter biosynthesis; neurotransmitter transport; nucleic acid sequence; synaptic vesicles; transcription factor

The long-term objective of the proposed research is to understand better the cellular processes in acetylcholine biosynthesis, secretion, and neuronal function. Despite intensive study of the cholinergic nervous system, it is poorly understood at the molecular level. With the complete nucleotide sequence and a functional promoter for the C. elegans acetylcholine biosynthesis and synaptic vesicle transporter genes (cha-1 and unc-17 respectively), we now possess the tools to conduct a powerful molecular and genetic analysis of cholinergic function in nematodes. The organization of these two genes in a eukaryotic operon is quite unique; the unc-17 coding sequence is completely nested within the first intron of cha-1, and their mRNAs are produced by alternative splicing of a common precursor RNA. This overall structure is conserved from nematodes to mammals, indicating that it may be crucial for regulation. The research proposed herein seeks to understand the regulation of cha-1/unc-17 expression, as well as the function of specific subsets of cholinergic neurons in C. elegans behavior.

拟议研究的长期目标是更好地了解 乙酰胆碱生物合成，分泌和 神经元功能。 尽管深入研究了胆碱能神经 系统，它在分子水平上的理解很少。 与 C的完整核苷酸序列和C的功能启动子。 秀丽隐杆线虫乙酰胆碱生物合成和突触小囊泡转运蛋白 基因（分别CHA-1和UNC-17），我们现在拥有工具 对胆碱能进行强大的分子和遗传分析 在线虫中的功能。 这两个基因的组织 真核操纵子非常独特。 UNC-17编码序列是 完全嵌套在CHA-1的第一个内含子中，它们的mRNA是 通过替代剪接的常见前体RNA产生。 这 从线虫到哺乳动物的整体结构是保守的，表明 这对于调节可能至关重要。 本文提出的研究 试图了解CHA-1/UNC-17表达的调节 作为秀丽隐杆线虫中胆碱能神经元特定子集的功能 行为。