POLYMORPHIC UPSTREAM STRUCTURE & PFG 27/25 GENE CONTROL

多态上游结构

• 批准号: 2687342
• 负责人: LAURA G POLOGE
• 金额: $ 33.55万
• 依托单位: NEW YORK UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-05-01 至 2002-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2687342
• 关键词: Plasmodium falciparum; developmental genetics; gene expression; genetic mapping; genetic polymorphism; genetic transcription; microorganism culture; microorganism genetics; nucleic acid hybridization; nucleic acid probes; nucleic acid sequence; polymerase chain reaction; protein structure; pulsed field gel electrophoresis; regulatory gene; sex differentiation; southern blotting; transfection

DESCRIPTION (Adapted from the Applicant's Abstract): The Plasmodium falciparum very early gametocyte stage gene, Pfg27/25, has a polymorphic locus upstreasm of the coding sequences which is surrounded by conserved sequences. The structure of the polymorphic region specifies accurate or altered blood stage specific transcription of the gene. The polymorphic structures arise by an as yet uncharacterizes DNA rearrangement. The overall objectives of this proposal are to determine the detailed structure of this locus in laboratory isolates that display different blood satge specific transcription patterns for the Pfg17/25 gene, and the extent to which these polymorphisms occur in nature. The 5' terminus of the Pfg27/25 mRNAs will also be determined to see where the transcription start sites occur with respect to the boundaries of the polymorphic domain.

描述（改编自申请人的摘要）：疟原虫 恶性疾病非常早期的配子细胞阶段基因PFG27/25具有多态性 编码序列的上游位点，该序列被保守所包围 序列。 多态区域的结构指定准确或 基因的血液阶段特异性转录改变。 多态性 结构尚未使DNA重排的尚未表达。 这 该提案的总体目标是确定详细的结构 在实验室分离株中的这个基因座，显示出不同的血液 PFG17/25基因的特定转录模式以及 这些多态性在自然界中出现。 PFG27/25的5'末端 我还将确定mRNA以查看转录启动站点的位置 发生关于多态域的边界。