MOLECULAR CHARACTERIZATION OF ROCHALIMAEA ANTIGENS

Rochalimaea 抗原的分子特征

• 批准号: 2672522
• 负责人: BURT E ANDERSON
• 金额: $ 10.13万
• 依托单位: UNIVERSITY OF SOUTH FLORIDA
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-07-01 至 2000-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2672522
• 关键词: Rickettsia; Rickettsiales disease; bacterial antigens; blood vessel disorder; epitope mapping; fusion gene; genetic library; human tissue; lymphatic disorder; molecular cloning; nucleic acid sequence; Rickettsia; Rickettsiales disease; bacterial antigens; blood vessel disorder; epitope mapping; fusion gene; genetic library; human tissue; lymphatic disorder; molecular cloning; nucleic acid sequence

Bacillary angiomatosis (BA) is a vascular proliferative disease of the skin and visceral organs initially described in HIV-infected patients. The etiologic agents of this disease are two species of highly fastidious, and closely related bacteria, Rochalimaea henselae and R. quintana. Since BA is a newly described disease and R. henselae is a recently described organism, the important, immunologically active antigens of these organisms have not yet been defined. The long range goals of the proposed studies are to identify immunologically active proteins of R. henselae and R. quintana and to characterize their roles in the immune response to Rochalimaea infection. These studies are designed to produce new information about the antigenic composition of Rochalimaea and the humoral immune response to infection with Rochalimaea. By analyzing specific Rochalimaea antigens (and their genes) at the molecular level, we should be able to produce valuable reagents for the diagnosis of Rochalimaea infections. Specific aims of the project are: 1) identify the immuno-dominant antigens of R. henselae and R. quintana; 2) cloning and sequencing the genes coding these major antigenic proteins; and 3) evaluating the recombinant antigens as diagnostic reagents for BA and cat-scratch disease (CSD). The basic approach will be to screen recombinant gene libraries of R. henselae and R. quintana for expression of antigens reactive with human sera from patients with BA and GSD. Immunoreactive clones will be subcloned, and sequenced and these recombinants will be sources of individual proteins for evaluation as immunodiagnostic reagents and studies to analyze their roles in the immune response to infection with Rochalimaea.

杆菌血管瘤病（BA）是一种血管增生性疾病 皮肤和内脏器官最初在HIV感染的患者中描述。这 该疾病的病因学剂是两种非常挑剔的物种， 密切相关的细菌，Rochalimaea Henselae和R. Quintana。自BA以来 是一种新描述的疾病，henselae是最近描述的 有机体，这些重要的，免疫学活跃的抗原 生物尚未定义。拟议的远程目标 研究旨在鉴定henselae和Henselae和 R. quintana并表征其在免疫反应中的作用 rochalimaea感染。 这些研究旨在产生新的 有关Rochalimaea和体液的抗原组成的信息 对rochalimaea感染的免疫反应。通过分析特定 rochalimaea抗原（及其基因）在分子水平上，我们应该 能够生产有价值的试剂以诊断rochalimaea 感染。 该项目的具体目的是：1）确定免疫主导抗原 R. Henselae和R. Quintana; 2）克隆和测序基因编码 这些主要的抗原蛋白； 3）评估重组抗原 作为BA和Cat-Scratch疾病（CSD）的诊断试剂。基本 方法是筛选henselae和Henselae的重组基因库 R. quintana用于表达抗原与人血清反应性的抗原 BA和GSD的患者。免疫反应性克隆将被亚克隆，并 测序和这些重组将是单个蛋白质的来源 评估作为免疫诊断试剂和研究以分析其 在对rochalimaea感染的免疫反应中的作用。