EFFECTS OF UV RADIATION ON UNICELLULAR ALGAE

紫外线辐射对单细胞藻类的影响

• 批准号: 6240071
• 负责人: ROBERT A KINZIE
• 金额: $ 1.52万
• 依托单位: UNIVERSITY OF HAWAII AT MANOA
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-04-01 至 1998-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6240071
• 关键词: algae; alternatives to animals in research; light adverse effect; photobiology; photosynthesis; photosystem; plant growth /development; species difference; ultraviolet radiation

The overall aim of this work is to investigate effects of current and predicted levels of long and middle range (UV-A, 320-400nm and UV-B, 280- 320 nm) ultraviolet radiation on photosynthetic systems, particularly phytoplankton and reef building corals. MBRS students have in the past and will continue to actively participate in several aspects of this work. The specific objectives of the MBRS student's research consist of training not only in proper use of the equipment, but more importantly in the ways of asking scientific questions and developing research projects to answer the questions so formulated. Specific research goals are 1) To elucidate effects of chronic exposure to UV-B on single celled algae, 2) To determine if there are species specific difference in tolerance to UV that might result in shifts in community composition under UV stress. The exposure experiments use two UV sources: 1) natural solar radiation filtered, when experimental protocol requires, through selective filters and 2) artificial radiation produced by our Xenon arc "solar simulator". The MBRS students receive training in quantification of light and UV radiation and in designing and building incubation systems. The effects of exposure are monitored through analysis of growth rates and composition of both photosynthetic and UV-absorbing pigments. Additionally photosynthetic capacity is measured before and after exposure to UV using 14C fixation. Effects of UV stress on species composition are initially being followed through two-species competition experiments run under differing levels of UV radiation.

这项工作的总体目的是调查当前和 预测长和中间范围的水平（UV-A，320-400NM和UV-B，280- 320 nm）光合系统上的紫外线辐射，特别是 浮游植物和礁石建筑珊瑚。 MBRS学生过去有 并将继续积极参与其中的几个方面 工作。 MBRS学生研究的具体目标包括 不仅要适当使用设备，而且更重要的是 以提出科学问题和发展研究的方式 项目以回答如此提出的问题。 具体的研究目标是1）阐明慢性暴露的影响 到单细胞藻类上的UV-B，2）确定是否存在物种 对紫外线的耐受性的具体差异可能会导致变化 紫外线压力下的社区组成。 暴露实验使用两个紫外线来源：1）天然太阳辐射 通过选择性过滤器进行实验协议时过滤 2）我们的氙弧“太阳模拟器”产生的人造辐射。 MBRS学生接受量化光和紫外线的培训 辐射以及设计和建筑孵化系统。 通过分析增长率来监测暴露的影响 以及光合作用和吸收紫外线的色素的组成。 另外，在光合作用能力之前和之后测量 使用14C固定接触UV。 紫外应力对物种的影响 最初通过两种类型的竞争遵循构图 实验在不同水平的紫外线辐射下进行。