CATION TRANSPORT IN DOG ERYTHROCYTES

狗红细胞中的阳离子转运

• 批准号: 6239994
• 负责人: EARL DIXON
• 金额: $ 12.63万
• 依托单位: TUSKEGEE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-06-01 至 1998-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6239994
• 关键词: SDS polyacrylamide gel electrophoresis; antiport; bioenergetics; biological transport; calcium channel; cell morphology; cell water; dogs; electron microscopy; erythrocyte membrane; erythrocytes; gel electrophoresis; membrane permeability; membrane proteins; membrane transport proteins; protein kinase; sodium channel

The major objectives of this research project are to determine the mechanisms responsible for volume regulation in dog regulation in dog erythrocytes. When dog erythrocytes are exposed to anastomotic media, the is an activation of either the Na/H antiporter or K-C1 cotransport systems to return the cell back to its normal volume. Studies in our laboratory have been designed to quantitate these pathways and determine the stoichimetry, inhibitors and activators of these transport systems. Recent studies have been design to determine the heterogeneity of cells have been assayed for their ion and water content as well as their specific density. Future studies will be designed to determine the transport properties of these cell fractions in order to explain the physiological mechanism responsible for these observations. It is anticipated that the results of these studies will allow us to determine the mechanisms responsible for cell volume regulation in vivo.

该研究项目的主要目标是确定 负责狗调节体积调节的机制 红细胞。 当狗红细胞暴露于吻合介质时 这是Na/H抗植物或K-C1的激活 将单元返回其正常体积的系统。 我们的研究 实验室旨在量化这些途径并确定 这些运输系统的石化，抑制剂和激活因子。 最近的研究是为了确定细胞的异质性 已经被测定了其离子和水含量以及他们的 比重。 未来的研究将旨在确定 这些细胞分数的传输特性是为了解释 负责这些观察的生理机制。 这是 预计这些研究的结果将使我们能够确定 负责体内细胞体积调节的机制。