RETROVIRAL ETIOLOGY OF WALLEYE EPIDERMAL HYPERPLASIA

白斑眼表皮增生症的逆转录病毒病因学

• 批准号: 2599580
• 负责人: JAMES W CASEY
• 金额: $ 10万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-15 至 1999-09-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2599580
• 关键词: RNA splicing; Retroviridae; Retroviridae disease; antiviral antibody; complementary DNA; disease /disorder etiology; fish; immunogenetics; northern blottings; nucleic acid probes; nucleic acid sequence; polymerase chain reaction; skin hyperplasia; tissue /cell culture; viral carcinogenesis; virus genetics; virus protein

DESCRIPTION: The role of two retroviruses, walleye epidermal hyperplasia virus types 1 and 2 (WEHV1 and WEHV2), in the etiology of discrete epidermal hyperplasia in walleyes will be examined. These hyperplastic lesions resemble psoriatic plaques in humans and skin lesions that appear on HIV Tat transgenic mice. WEHV1 and 2 resemble complex retroviruses and it is hypothesized that the expression of accessory genes is involved in cellular proliferation. To study the induction and regression of walleye epidermal hyperplasia transcription maps will be developed to examine splicing patterns, exon usage, and cDNA complexity. Antibodies to viral structural and accessory proteins will be produced to study the WEHV replicative cycle in cultured cells. Hyperplasia induction and regression will be followed with nucleic acid probes and antibodies to identify viral gene products implicated in these processes.

描述：两种逆转录病毒的作用，角膜白斑表皮增生 病毒类型1和2（WEHV1和WEHV2），在离散表皮的病因中 将检查角膜白斑的增生。 这些增生病变 类似于人类和皮肤病变中出现在HIV TAT上的银屑病斑块 转基因小鼠。 WEHV1和2类似于复杂的逆转录病毒，它是 假设辅助基因的表达与细胞有关 增殖。 研究角膜表皮的诱导和回归 将开发增生转录图以检查剪接 模式，外显子使用和cDNA复杂性。 病毒结构的抗体 并将生产辅助蛋白来研究WEHV复制周期 在培养的细胞中。 将遵循增生诱导和回归 用核酸探针和抗体鉴定病毒基因产物 与这些过程有关。