SYNTAXINS AND MEMBRANE TRAFFICKING

突触连接蛋白和膜运输

基本信息

批准号：
2459548
负责人：
MARK K BENNETT
金额：
$ 23.76万
依托单位：
UNIVERSITY OF CALIFORNIA BERKELEY
依托单位国家：
美国
项目类别：
财政年份：
1994
资助国家：
美国
起止时间：
1994-08-01 至 1999-07-31
项目状态：
已结题

项目摘要

Membrane trafficking contributes to the intracellular organization of all eukaryotic cells, and to the specialized functions of many cell types. The membranous compartments of the secretory and endocytic pathways exhibit distinct biochemical properties related to the specific functions that they perform. This intracellular organization is maintained, in part, by transport vesicles that shuttle membrane and soluble cargo from one intracellular compartment of another. In specialized cell types, such as epithelial cells, vesicular trafficking plays and important role in the maintenance of cell surface polarity that is essential for vectorial transport and secretory functions. Furthermore, the regulation of certain membrane trafficking events, for example those underlying the regulated secretion of neurotransmitter at the synapse, play important roles in intercellular communication. The long-term objective of the proposed research is an understanding of the molecular mechanisms responsible for these intracellular membrane trafficking events. Such an understanding will ultimately provide insight into a wide variety of cellular processes. The strategy to be employed in approaching this objective is the biochemical and functional characterization of a family of proteins, the syntaxins, that have been proposed to act as receptors for intracellular membrane transport vesicles. Particular emphasis will be focused on the role of syntaxin 1 in synaptic vesicle docking and fusion. The proposed research will address the following specific aims: 1. Biochemical characterization of the interaction between membrane of the syntaxin family and other specific components of the intracellular membrane trafficking machinery. 2. Identification of the signal(s) within the syntaxins responsible for localization to different intracellular membrane compartments. 3. Functional characterization of the role of syntaxins in constitutive and regulated secretion from PC12 cells.

膜贩运有助于所有人的细胞内组织真核细胞，以及许多细胞类型的专业功能。分泌和内吞途径的膜隔室具有与特定功能相关的不同生化特性他们执行。维持这个细胞内组织部分，通过运输囊泡，从膜和可溶性货物中驶出另一个细胞内室。在专门的细胞类型中作为上皮细胞，囊泡运输在维持细胞表面极性，这对于媒介物必不可少运输和分泌功能。此外，某些人的调节膜贩运事件，例如那些受监管的人神经递质在突触中的分泌，在细胞间沟通。提议的长期目标研究是对负责分子机制的理解这些细胞内膜贩运事件。这样的理解最终将提供有关各种蜂窝的洞察力过程。实现这一目标的策略是蛋白质家族的生化和功能表征，提议作为受体的语法素细胞内膜转运囊泡。特别强调将是专注于语法1在突触囊泡对接和融合中的作用。拟议的研究将解决以下具体目的： 1。膜之间相互作用的生化表征细胞内的语法素家族和其他特定成分膜贩运机械。 2。识别负责的语法中信号定位到不同细胞内膜室。 3。语法在本构中的作用的功能表征并调节PC12细胞的分泌。

项目成果

期刊论文数量（6）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Characterization of SNARE protein expression in beta cell lines and pancreatic islets.

DOI：
10.1210/endo.137.4.8625909
发表时间：
1996-04
期刊：
Endocrinology
影响因子：
4.8
作者：
M. Wheeler;L. Sheu;M. Ghai;A. Bouquillon;G. Grondin;U. Weller;A. Beaudoin;M. Bennett;W. Trimble;H. Gaisano
通讯作者：
M. Wheeler;L. Sheu;M. Ghai;A. Bouquillon;G. Grondin;U. Weller;A. Beaudoin;M. Bennett;W. Trimble;H. Gaisano

Protease resistance of syntaxin.SNAP-25.VAMP complexes. Implications for assembly and structure.

Syntaxin.SNAP-25.VAMP 复合物的蛋白酶抗性。