INSTABILITY OF SIMPLE REPETITIVE DNA SEQUENCES IN YEAST

酵母中简单重复 DNA 序列的不稳定性

• 批准号: 2415280
• 负责人: THOMAS PETES
• 金额: $ 18.17万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-05-01 至 1998-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2415280
• 关键词: DNA; Escherichia coli; Saccharomyces cerevisiae; chemical stability; fungal genetics; genetically modified animals; laboratory mouse; nucleic acid repetitive sequence; nucleic acid structure; plasmids

DESCRIPTION (adapted from investigator's abstract): All eukaryotes contain tracts of simple repetitive DNA, in which a single base or a small number of bases is repeated multiple times. Repetitive tracts tend to be unstable, increasing or decreasing in size at a rate that is much higher than that observed for "normal" non-repeated DNA sequences. Using plasmids with insertions of repetitive DNA in the coding sequence of selectable genes, tract instability can be assayed in the yeast, Saccharomyces cerevisiae. The proposed experiments examine some properties of this type of genetic instability. First, mutants of yeast that have increased levels of instability will be examined. This study should yield evidence relevant to the mechanisms of tract instability. Second, the effects of altering the sequence or the length of the repetitive DNA tracts will be examined. Third, it will be determined whether alterations in tract length are associated with crossing-over. Fourth, a transgenic mouse in which the in vivo instability of simple repeats can be monitored will be developed. Fifth, a search will be made for mechanisms allowing large expansions of simple repeats in yeast cells since most of the alterations detected to date represent small changes.

描述（改编自研究者的摘要）：所有真核生物 含有大量简单的重复DNA，其中单个碱基或一个 少量碱基重复多次。 重复的小册子 往往不稳定，尺寸以一定的速度增加或减小 远高于观察到的“正常”非重复 DNA 序列。 使用在编码序列中插入重复 DNA 的质粒 可选择的基因，可以在酵母中测定肠道不稳定性， 酿酒酵母。 拟议的实验检查了一些 这种遗传不稳定性的特性。 一、酵母突变体 那些增加了不稳定程度的因素将受到审查。 这项研究 应提供与束不稳定机制相关的证据。 其次，改变序列或长度的影响 将检查重复的 DNA 片段。 三、将确定 束长度的改变是否与交叉有关。 四、转基因小鼠体内不稳定性简单 将开发可监测的重复。 第五，将进行搜索 专为允许酵母中简单重复的大量扩增而设计的机制 细胞，因为迄今为止检测到的大多数改变都代表很小 变化。