CELL FREE APOPTOSIS--FACTORS AND MECHANISMS

无细胞凋亡--因素和机制

• 批准号: 2022794
• 负责人: DONALD DAVID NEWMEYER
• 金额: $ 16.4万
• 依托单位: LA JOLLA INSTITUTE FOR IMMUNOLOGY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-01-01 至 2000-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2022794
• 关键词: DNA; Xenopus oocyte; affinity chromatography; alternatives to animals in research; antibody; apoptosis; biological models; cell cycle; cell free system; ceramides; cytoplasm; enzyme activity; model design /development; molecular cloning; protease inhibitor; protein degradation; protein kinase; protooncogene

DESCRIPTION (adapted from investigator's abstract): The investigator has developed a novel cell -free system based on extracts from eggs of the frog, Xenopus laevis. Such egg extracts are able to reproduce many of the features of apoptosis, including chromatin condensation, endonucleolytic DNA cleavage and the shrinkage and fragmentation of the nuclei. Lysates from baculovirus-infected cells expression the oncogene bcl-2 can suppress nuclear fragmentation. This system provides an excellent means to dissect the molecular mechanisms of apoptosis and protective function of bcl-2. The applicant proposes to identify and characterize some of the cytoplasmic events of apoptosis. Such cell-free systems offer the opportunity to reconstitute the apoptotic process from fractionated components.

描述（根据调查员的摘要改编）：调查员有 基于青蛙卵的提取物开发了一种新型的无细胞系统， Xenopus laevis。 这样的鸡蛋提取物能够复制许多 细胞凋亡的特征，包括染色质凝结，内核解释性DNA 细胞核的裂解以及收缩和碎片化。 裂解物 杆状病毒感染的细胞表达癌基因Bcl-2可以抑制 核碎裂。 该系统提供了剖析的绝佳方法 Bcl-2的凋亡和保护功能的分子机制。 这 申请人建议识别和表征一些细胞质 凋亡事件。 这样的无单元系统为 从分馏组件中重建凋亡过程。