DEVELOPMENT OF CILIARY EPITHELIUM

睫状上皮的发育

• 批准号: 2614261
• 负责人: DAVID CY BEEBE
• 金额: $ 23.62万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-04-01 至 2003-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2614261
• 关键词: Xenopus oocyte; animal genetic material tag; anterior chamber; biomarker; cell differentiation; chick embryo; collagen; corneal endothelium; epithelium; gel mobility shift assay; gene expression; gene targeting; genetic regulatory element; histogenesis; homeobox genes; human genetic material tag; in situ hybridization; iris; laboratory mouse; lens; messenger RNA; migration inhibition factor; transcription factor; uvea ciliary body; vertebrate embryology

DESCRIPTION: During the past grant period the PI has shown that the normal differentiation of the tissues of the anterior segment of the eye is regulated by the lens. Removal of the lens prevent the normal differentiation of the ciliary epithelium, cornea, iris and trabecular meshwork. Transplantation of the lens to the posterior of the optic cup causes cells that would have become retina to differentiate into ciliary epithelium and iris. Transplantation of a second lens to the anterior of the optic cup induces the formation of a second anterior chamber, iris and ciliary epithelium with ciliary folds. in the current proposal he will complete experiments demonstrating the forces responsible for the formation of the ciliary folds, determine the stages at which the lens produces the factor(s) necessary for organizing the anterior segment, identify the factors produced by the lens required for its organizing activity, determine the role of the lens in regulating the expression of mRNAs for several transcription factors known or believed to be important in the development of the anterior segment and identify cis-acting elements and transcription factors involved in regulating the synthesis of collagen IX, a major protein product of the ciliary epithelium. These studies should reveal the regulatory network required for the normal development of the tissues of the anterior segment. It is also likely that this work will identify candidate genes and the regulatory network involved in hereditary anterior segment dysgenesis and congenitalgalucoma.

描述：在过去的赠款期间，PI表明正常 眼睛前部的组织的分化为 由镜头调节。 去除镜头预防正常 睫状上皮，角膜，虹膜和小梁的分化 网眼。 将镜头移植到光杯的后部 引起将成为视网膜分化为睫状的细胞 上皮和虹膜。 将第二个镜头移植到 光杯诱导第二个前室，虹膜和 睫状上皮带有睫状褶皱。 在当前的提议中 完整的实验证明了负责形成的力 在睫状褶皱中，确定镜头产生的阶段 组织前部所需的因素，确定 由其组织活动所需的镜头产生的因素，确定 镜头在调节mRNA表达中的作用 已知或认为在开发中很重要的转录因子 前部段并识别顺式作用元素和转录 调节胶原蛋白IX的合成涉及的因素，这是一种主要蛋白 睫状上皮的产物。 这些研究应该揭示 正常发育的组织需要的监管网络 前部。 这项工作也可能会确定候选人 基因和遗传前部涉及的监管网络 发育不全和先天性gal瘤。