DROSOPHILA OOGENESIS AND OVARIAN TUMOR FORMATION

果蝇卵发生和卵巢肿瘤形成

• 批准号: 2684965
• 负责人: RODNEY N NAGOSHI
• 金额: $ 18.59万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-08-01 至 2000-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2684965
• 关键词: Drosophilidae; RNA binding protein; RNA splicing; carcinogenesis; cell differentiation; developmental genetics; gel mobility shift assay; gene expression; genetic mapping; genetic regulatory element; molecular cloning; nucleic acid sequence; nucleic acid structure; oncogenes; oogenesis; ovary neoplasms; sex determination; transcription factor; transfection

DESCRIPTION: This application is investigating the function of the ovarian tumor group genes in Drosophila oogenesis. The long-term objective is to elucidate the pathways controlling female germline differentiation. Mutants of the ovarian tumor (otu) gene display allele specific defects ranging from agametic ovaries to neoplastic growth, the least severe being incomplete maturation of the oocytes. Otu gene expression is required only in the germline. Alternative splicing of Otu transcripts results in two isoforms of 98 and 104 kd, differing by 42- aa. Rescue experiments with the 98 kd isoform causes otu mutant agametic ovaries to develop tumorous egg chambers suggesting that proliferation is normal but differentiation of the female germline is abnormal. In addition, an otu mutation which blocks the alternative splicing pathway yields only the 98kd isoform, and this mutant also has the tumorous egg chamber phenotype.

描述：此应用程序正在研究 果蝇中的卵巢肿瘤基因。 长期 目的是阐明控制女性种系的途径 分化。 卵巢肿瘤（OTU）基因的突变体显示等位基因 特定缺陷范围从果胶卵巢到肿瘤生长， 卵母细胞不完全成熟。 OTU基因 仅在种系中需要表达。 OTU的替代剪接 转录本导致两种同工型为98和104 kd，不同于42-- aa。 98 kD同工型的救援实验会导致OTU突变体 开发肿瘤卵室的agametic卵巢表明 增殖是正常的，但女性种系的差异是 异常。 另外，可阻塞替代方案的OTU突变 剪接途径仅产生98KD同工型，并且该突变体也具有 肿瘤卵室表型。