PROTEIN/PROTEIN AND PROTEIN/NUCLEOTIDE INTERACTIONS IN MICROTUBULE ASSEMBLY

微管组装中的蛋白质/蛋白质和蛋白质/核苷酸相互作用

• 批准号: 2463720
• 负责人: E HAMEL
• 金额: --
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2463720
• 关键词: antimitotics; antineoplastics; chemical stability; chemical structure function; drug design /synthesis /production; drug screening /evaluation; guanine nucleotides; intermolecular interaction; microtubule associated protein; microtubules; nucleotide analog; protein purification; protein structure function; tubulin

The rational development of new antineoplastic agents directed against tubulin, a protein critical for cell division, requires greater understanding of the interaction between the polypeptide subunits of tubulin, its two tightly bound guanine nucleotides, and microtubule-associated proteins (MAPS). The effects of nucleotides on the stability of microtubules continued to be examined, as were conditions to optimize the separation of alpha-tubulin and beta-tubulin on a preparative scale. The purification of a microtubule-associated protein which causes the formation of microtubule bundles continued to progress, and a project to introduce potentially antimitotic nucleotide analogs into cells continued. Studies with 1-thiophosphate analogs of GDP, GTP, 2',3'-dideoxyguanosine nucleotides were begun. A project to localize the nonexchangeable GTP binding site was continued.

针对的新抗塑料剂的合理发展 微管蛋白是一种至关重要的细胞分裂的蛋白质，需要更大 了解多肽亚基之间的相互作用 微管蛋白，其两个紧密结合的鸟嘌呤核苷酸和 微管相关蛋白（地图）。 核苷酸对 微管的稳定性继续检查 优化α-微管蛋白和β-微管蛋白分离的条件 在准备量表上。 微管相关的纯化 导致微管束的形成的蛋白质继续 进度和一个引入潜在抗魔法核苷酸的项目 类似于细胞的类似物继续。 研究1-位磷酸类似物的研究 GDP，GTP，2'，3'-二氧鸟苷核苷酸开始。 一个项目 继续定位不合规的GTP结合位点。