ABSORPTION OF ELLAGITANNINS FROM DIETARY SOURCES

从膳食中吸收鞣花单宁

• 批准号: 2012139
• 负责人: DANIEL W NIXON
• 金额: $ 3.89万
• 依托单位: MEDICAL UNIVERSITY OF SOUTH CAROLINA
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-05-15 至 1999-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2012139
• 关键词: cancer prevention; chemical structure function; chemoprevention; clinical research; dietary constituent; fruit; gastrointestinal nutrient absorption; high performance liquid chromatography; human subject; infrared spectrometry; metabolism; nuclear magnetic resonance spectroscopy; nutrition aspect of cancer; nutrition related tag; pharmacokinetics; serology /serodiagnosis; tannins; urinalysis

DESCRIPTION: The long term goal of this project is to determine if hydrolyzable ellagitannins present in dietary sources prevent cancer in humans. The more immediate purpose of this project is to assess the a b s o r ption and metabolism of hydrolyzable ellagitannins present in raspberries, strawberries and walnuts by the human digestive system. We believe that the hydrolyzable ellagitannins are absorbed into the human blood stream and metabolized after raspberries, strawberries and walnuts are consumed. We also believe that these ellagitannins and metabolites can be identified and quantified. The purpose for doing this is to identify biologically active ellagitannins and metabolites for further cell and animal chemoprevention studies. This study will also identify which dietary source delivers the highest concentration of biologically active ellagitannins to the blood stream, which will be useful for continuing human chemoprevention clinical trials. In order to identify and quantify the ellagitannins absorbed into the blood stream after raspberries, strawberries, and walnuts are consumed, thirty healthy volunteers will be broken into three equal groups and admitted to the MUSC General Clinical Research Center. After baseline urine and serum samples are obtained, the volunteers will be fed a known quantity of either raspberries, strawberries or walnuts under controlled conditions. Urine will be collected and a serial blood draw will be taken from each volunteer over the 24-hour study period. Known ellagitannins will then be identified and quantified in the blood and urine by HPLC. Unknown ellagitannins and ellagitannin metabolites will be isolated by HPLC and submitted for Mass Spectrum (FAB), NMR, UV and FR-IR analysis so that structural determinations can be made.

描述：该项目的长期目标是确定是否 膳食来源中存在的可水解鞣花单宁可预防癌症 人类。 该项目更直接的目的是评估 a b s o 覆盆子中存在的可水解鞣花单宁的作用和代谢， 草莓和核桃被人体消化系统吸收。 我们相信 可水解的鞣花单宁被人体血液吸收 食用覆盆子、草莓和核桃后代谢。 我们 还相信这些鞣花单宁和代谢物可以被识别并 量化。 这样做的目的是识别生物活性 用于进一步细胞和动物化学预防的鞣花单宁和代谢物 研究。 这项研究还将确定哪种饮食来源可以提供 血液中生物活性鞣花单宁的浓度最高 流，这将有助于持续的人类化学预防临床 试验。 为了鉴定和定量吸收的鞣花单宁 食用覆盆子、草莓和核桃后的血液流动， 三十名健康志愿者将被分成三个相等的组 被MUSC普通临床研究中心录取。 基线尿液后 获得血清样本后，将给志愿者喂食已知量 在受控条件下种植覆盆子、草莓或核桃。 将收集尿液并对每个人进行连续抽血 24小时学习期间的志愿者。 已知的鞣花单宁将是 通过 HPLC 在血液和尿液中进行鉴定和定量。 未知 将通过 HPLC 分离鞣花单宁和鞣花单宁代谢物 提交进行质谱 (FAB)、NMR、UV 和 FR-IR 分析，以便 可以做出结构性决定。