ULTRASTRUCTURAL CORRELATES OF DEVELOPMENT IN TASTE BUDS

味蕾发育的超结构关联

• 批准号: 3732251
• 负责人: JOHN KINNAMON
• 金额: --
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3732251
• 关键词: afferent nerve; amiloride; antigens; cell type; chemical stimulation; computer simulation; developmental neurobiology; electrophysiology; gap junctions; high voltage electron microscopy; immunocytochemistry; ion transport; laboratory rat; protein structure function; sodium channel; synapses; taste buds; transmission electron microscopy; voltage /patch clamp; voltage gated channel

The purpose of this application is to elucidate the structural and functional bases for development of the gustatory system. The proposed project will use ultrastructural, immunohistochemical, immunocytochemical and electrophysiological approaches to correlate structure with function in developing taste buds of the rat. The rat was selected for this study because the gustatory system develops during the first few weeks after birth. Specific features of developing taste buds will be studied at selected ages, including: formation of the taste pore, types of taste cells present, time of first synapse formation and the types of taste cells involved, specificity of synaptic connections within the taste buds, immunoreactivity to antibodies directed against neuronal, growth- related and other specific proteins. The patch-clamp technique will be used to monitor the first expression and development of specific ion channels, amiloride sensitivity, and responses to chemical stimulation in developing gustatory receptor cells. The results of these studies will help answer fundamental questions regarding the development of gustation in mammals and how the nervous system interacts with taste buds during ontogeny. An understanding of the process of development in the gustatory system will also provide valuable information that may enhance our understanding of the regeneration of taste buds and other portions of the nervous system after physical trauma or disease states.

本申请的目的是阐明结构和 味觉系统发育的功能基础。 拟议的 项目将使用超微结构、免疫组织化学、免疫细胞化学 和电生理学方法将结构与功能关联起来 促进大鼠味蕾的发育。 大鼠被选用于本研究 因为味觉系统是在出生后的最初几周内发育的 出生。 正在研究味蕾发育的具体特征 选定的年龄，包括：味孔的形成、味觉类型 存在的细胞、首次突触形成的时间以及味觉的类型 涉及的细胞，味觉内突触连接的特异性 芽，针对针对神经元的抗体的免疫反应性，生长- 相关的和其他特定的蛋白质。 膜片钳技术将是 用于监测特定离子的首次表达和发育 通道、阿米洛利敏感性和对化学刺激的反应 味觉受体细胞的发育。 这些研究的结果将有助于回答基本问题 关于哺乳动物味觉的发展以及神经如何 系统在个体发育过程中与味蕾相互作用。 的理解 味觉系统的发展过程也会提供 有价值的信息可以增强我们对事物的理解 味蕾和神经系统其他部分的再生 身体创伤或疾病状态。