AC IMPEDANCE SPECTROSCOPY FOR HIV DNA DETECTION

用于 HIV DNA 检测的交流阻抗光谱

• 批准号: 2005782
• 负责人: MICHAEL T CARTER
• 金额: $ 10万
• 依托单位: ELTRON RESEARCH, INC.
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-30 至 1999-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2005782
• 关键词: artificial intelligence; bioengineering /biomedical engineering; biomedical equipment development; electrical impedance; electrochemistry; electrodes; human immunodeficiency virus; interferometry; nucleic acid hybridization; spectrometry; virus DNA

DESCRIPTION: (Adapted from the applicant's abstract) This project proposes a biosensor for the detection of DNA fragments specific to HIV. The sensing element will be an interdigitated array (IDA) having single stranded DNA immobilized between the array's digits. Immobilized DNA strands will be complimentary to a conserved sequence in the HIV target thus allowing selective detection. Electronics capable of measuring impedance changes will be the detection of the hybridization of complementary DNA strands. Impedance spectroscopy, i.e., multiple frequency impedance measurements, will be used to completely characterize the sensor's response. The resulting impedance spectrum will be treated by Neural Network Analysis (NNA) to isolate the hybridized DNA response from non-specifically adsorbed species and other matrix effects. The technological innovations which will result from the project include a sensitive and specific HIV DNA detection scheme which would be applicable to any DNA assay.

描述：（根据申请人的摘要改编）这个项目 提出了一种生物传感器，用于检测特异性HIV的DNA片段。 传感元素将是一个具有单个的互换阵列（IDA） 滞留的DNA固定在阵列的数字之间。固定的DNA 链将与HIV目标中的保守序列相称 因此允许选择性检测。能够测量的电子设备 阻抗变化将是检测的杂交 互补的DNA链。阻抗光谱，即多个 频率阻抗测量将用于完全表征 传感器的响应。由此产生的阻抗频谱将被处理 通过神经网络分析（NNA）分离杂交DNA响应 来自非特殊吸附物种和其他基质效应。这 该项目将导致的技术创新包括 敏感和特定的HIV DNA检测方案，适用 进行任何DNA分析。