MULTICENTER STUDIES OF DIET AND LIPOPROTEINS

饮食和脂蛋白的多中心研究

• 批准号: 2659291
• 负责人: PATRICIA J. ELMER
• 金额: $ 4.32万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-30 至 1998-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2659291
• 关键词: adult human (21+); antihypercholesterolemic agent; blood lipoprotein; cardiovascular disorder prevention; cholesterol; clinical trials; coronary disorder; diet therapy; dietary lipid; disease /disorder proneness /risk; eicosanoid metabolism; fatty acids; gender difference; hemostasis; high density lipoproteins; human subject; human therapy evaluation; nutrition related tag; racial /ethnic difference; saturated fatty acids; sex hormones; triglycerides

Lipid and hemostatic profiles have etiologic importance in atherogenesis and are influenced by diet. The proposed study addresses key unresolved issues in the effects of fat and other diet constituents on blood lipid (total cholesterol, LDL-C, HDL-C, triglycerides) and hemostatic responses (fibrinogen, factor VII, plasminogen activator inhibitor, Beta-thromboglobulin and platelet factor 4). The proposed research includes a series of tightly controlled human nutrition feeding studies conducted within a multicenter feeding trial mechanism with a common protocol. The focus of this project is to determine the optimal composition for a lipid lowering diet. An integrated series of five 6-month Latin square design feeding studies will be conducted. The diets to be tested in 4 experiments with 5-week feeding periods evaluate modifications to the American Heart Association Step 1 diet (30% of Kcal from fat) and the typical American diet (37% Kcal from fat). Contrasts of most interest are 1) high monounsaturated fatty acids (MUFA) versus high polyunsaturated fatty acids (PUFA) at 30% and 37% Kcal total fat, 2) increased soluble fiber (concentrated Beta-glucan) at two dosage levels and different levels of total fat, 3) increased n -3 PUFA at 2 dosages and at different levels of total fat, 4) increased trans fatty acids at 2 levels and at different levels of total fat. The fifth experiment with an 11-week diet period evaluates a combination of components to yield optimal lipid response based on results of the first 4 experiments. Proposed ancillary outcomes include: LDL susceptibility to oxidation, antioxidant status, mononuclear cell cholesterol and postprandial lipids and insulin. The investigative group has expertise in the areas of nutrition, feeding studies, lipid biochemistry, dietetics, hemostasis, biostatistics, epidemiology and metabolism. Diets will be fed by the University of Minnesota general Clinical Research Center (CRC), which has long experience with production and feeding of well-controlled research diets. Each experiment will be conducted with 20 women, ages 30-64, recruited using well-established advertising and referral procedures. The interdisciplinary components of the study will be coordinated by a local Executive Committee which meets regularly and interfaces with the national Steering Committee, Day-to-day operations will be handled by functional managers who report directly to the principal investigator and the project coordinator. These studies are consistent with the long terms research program of the investigators to elucidate relationships between diet and disease.

脂质和止血特性在动脉粥样硬化形成中具有重要的病因学意义 并且受饮食影响。 拟议的研究解决了尚未解决的关键问题 脂肪和其他饮食成分对血脂影响的问题 （总胆固醇、LDL-C、HDL-C、甘油三酯）和止血剂 反应（纤维蛋白原、因子 VII、纤溶酶原激活剂抑制剂、 β-血栓球蛋白和血小板因子 4)。 拟议的研究 包括一系列严格控制的人类营养喂养研究 在多中心喂养试验机制内进行，具有共同的 协议。 该项目的重点是确定最佳 用于降脂饮食的组合物。 五个为期 6 个月的拉丁方设计喂养研究的综合系列 将进行。 为期 5 周的 4 次实验中测试的饮食 喂养期评估美国心脏协会的修改 步骤 1 饮食（30% Kcal 来自脂肪）和典型的美国饮食（37% 来自脂肪的千卡）。 最感兴趣的对比是 1) 高单不饱和脂肪酸 (MUFA) 与高多不饱和脂肪酸 (PUFA) 分别为 30% 和 37% 千卡总脂肪，2) 增加可溶性纤维（浓缩β-葡聚糖） 两种剂量水平和不同水平的总脂肪，3) 增加 n -3 2 个剂量和不同总脂肪水平的 PUFA，4) 增加 2 个水平和不同总脂肪水平的反式脂肪酸。 这 第五个实验评估了为期 11 周的饮食组合 根据第一个结果产生最佳脂质反应的成分 4个实验。 提议的辅助结果包括： LDL 对氧化的敏感性， 抗氧化状态、单核细胞胆固醇和餐后脂质 和胰岛素。 该调查小组拥有营养、喂养等领域的专业知识 研究、脂质生物化学、营养学、止血、生物统计学、 流行病学和新陈代谢。 饮食将由大学提供 明尼苏达综合临床研究中心 (CRC) 具有良好控制的研究的生产和供给经验 饮食。 每个实验将针对 20 名年龄在 30-64 岁之间的女性进行， 使用完善的广告和推荐程序进行招聘。 该研究的跨学科组成部分将由一个 当地执行委员会定期举行会议并与 国家指导委员会，日常运作将由 直接向首席研究员报告的职能经理 和项目协调员。 这些研究与该研究所的长期研究计划是一致的。 研究人员阐明饮食与疾病之间的关系。