REDUCED GRAVITY--EFFECTS IN THE DEVELOPING NERVOUS SYST

重力降低——对神经系统发育的影响

• 批准号: 2445824
• 负责人: Richard S Nowakowski
• 金额: $ 28.27万
• 依托单位: UNIV OF MED/DENT NJ-R W JOHNSON MED SCH
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-09-30 至 1999-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2445824
• 关键词: brain cell; bromodeoxyuridine; cell cycle; cell differentiation; cell migration; cerebral cortex; developmental neurobiology; embryo /fetus cell /tissue; environmental adaptation; extraterrestrial environment; gestational age; gravity; laboratory mouse; laboratory rat; newborn animals; orientation; thymidine; tritium

DESCRIPTION: The applicant proposes to examine the effects of space flight and microgravity on the cells of the developing cerebral cortex. These studies will examine three cellular processes: 1) cell proliferation, 2) neuronal migration, and 3) neuronal differentiation, including dendrite/axon orientation and acquisition of appropriate neuronal circuitry. Two markers of cell proliferation (bromodeoxyuridine and tritiated thymidine) will be administered to pregnant rats on selected days during the development of the cerebral cortex. Short term analyses (sacrifice fetuses 2.5 hrs post-injection) will examine the responses of cell proliferation to space flight by examining changes in the number of proliferating cells or the length of the cell cycle. Intermediate-term effects will be assessed by sacrificing the fetal animals 1-3 days post-injection. The migratory fates of cells within the developing cerebral cortex will be examined. Long-term effects will be assessed by sacrificing animals that were treated as fetuses at different ages through 30 days after birth. The final position of cells born at particular ages will analyzed.

描述：申请人建议检查空间的影响 发育中的大脑皮层细胞上的飞行和微重力。 这些研究将检查三个细胞过程：1）细胞 增殖，2）神经元迁移和3）神经元分化， 包括树突/轴突方向和获取适当的 神经元电路。两个细胞增殖标记 （溴脱氧尿苷和trified胸苷）将被施用 在大脑发育期间选定的日子的怀孕大鼠 皮质。短期分析（牺牲胎儿2.5小时注射后） 将检查细胞增殖对太空飞行的反应 检查增殖细胞数量的变化或长度的变化 细胞周期。中期影响将由 注射后1-3天牺牲胎儿动物。迁徙 将检查发育中的脑皮质中细胞的命运。 长期影响将通过牺牲动物来评估 在出生后30天内被视为不同年龄的胎儿。这 在特定年龄段出生的细胞的最终位置将分析。