CONTROL OF PROLACTIN AND GH SECRETION FROM SINGLE CELLS

单细胞催乳素和 GH 分泌的控制

• 批准号: 2443998
• 负责人: L STEPHEN FRAWLEY
• 金额: $ 22.7万
• 依托单位: MEDICAL UNIVERSITY OF SOUTH CAROLINA
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-07-01 至 1999-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2443998
• 关键词: cell differentiation; gene expression; genetic promoter element; hormone regulation /control mechanism; laboratory rat; luciferin monooxygenase; plasmids; prolactin; reporter genes; secretion; single cell analysis; somatotropin; tissue /cell culture; transcription factor

DESCRIPTION (Adapted from the applicant's abstract): The mechanisms underlying hormonal gene expression and secretion are extremely complex, and key regulatory events are often separated in time. The understanding of these dynamic processes has been facilitated in part by the use of reverse hemolytic plaque assays which enable multiple measurements of hormone release from the same living endocrine cells. Progress in this area has been hindered by the lack of a method suitable for making dynamic measurements of gene expression. To monitor molecular events, the principal investigator has modified conventional luciferase reporter technology to enable "real-time" measurement of gene expression in individual endocrine cells. With this tool and the plaque assay, the principal investigator is able to pursue new levels of questions about gene expression and hormone secretion that were not addressable previously because of technical constraints. The specific aims of the current proposal are to 1) optimize and complete validation of the system for real-time measurement of gene expression; 2) establish the temporal relationship between gene expression and secretion in normal pituitary cells; and 3) elucidate the mechanisms governing the transdifferentiation of GH and PRL secreting cells. The results of these studies may greatly expand the fundamental knowledge about the relationship between hormonal gene expression and secretion in normal cells. This information may be critical to gaining an understanding about aberrant mechanisms leading to pathophysiologic states of hormone secretion.

描述（改编自申请人的摘要）：机制 潜在的激素基因表达和分泌非常复杂， 关键的监管事件通常会及时分开。理解 这些动态过程中的一部分是通过使用 反向溶血斑块测定法，可实现多次测量 激素从相同的活内分泌细胞释放。进步 缺乏适合制作的方法阻碍了区域 基因表达的动态测量。 为了监视分子事件，主要研究者已修改 传统的萤光素酶报告基技术启用“实时” 在个体内分泌细胞中基因表达的测量。与此 工具和斑块测定法，首席研究员能够追求 有关基因表达和激素分泌的新问题 由于技术限制，以前无法解决。这 当前建议的具体目的是1）优化和完成 实时测量基因表达的系统验证； 2）建立基因表达与 正常垂体细胞中的分泌； 3）阐明机制 管理GH和PRL分泌细胞的转分化。这 这些研究的结果可能会大大扩展基本知识 关于激素基因表达与分泌之间的关系 在正常细胞中。此信息可能对获得 了解导致病理生理的异常机制 激素分泌状态。