RESPONSIVE POLYMER ENGINEERED PROTEIN CONJUGATES

响应性聚合物工程蛋白缀合物

• 批准号: 2332021
• 负责人: ALLAN S HOFFM
• 金额: $ 19.06万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-02-01 至 1999-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2332021
• 关键词: CD antigens; G protein; X ray crystallography; acidity /alkalinity; allosteric site; binding proteins; biotin; chemical binding; chemical conjugate; covalent bond; cysteine; enzyme linked immunosorbent assay; flow cytometry; lymphocyte; molecular site; molecular weight; monoclonal antibody; nonvisual photosensitivity; polymerization; polymers; protein engineering; protein structure; radioassay; site directed mutagenesis; temperature

In this proposal, we propose to combine (a) the latest protein engineering techniques with (b) stimuli-responsive polymer compositions to produce (c) responsive polymer-engineered protein (RP-EP) conjugates that have unique behaviors and uses. More specifically, we propose to conjugate temperature-, pH- or light-sensitive oligomers by one of their terminal ends to a specific site near or within the active recognition site of selected proteins, and then to apply a temperature, pH or light stimulus to tern " (i.e., allow binding) or "off" (i.e., either reduce the binding affinity, prevent binding altogether, or eject a previously-bound receptor) the binding of the target ligand or cell to the proteins's recognition site. The protein could be an antibody, an enzyme, a hormone, an integrin, albumin, streptavidin, or any other recognition protein. The protein could be an antibody, an enzyme, a hormone, an integrin, albumin, streptavidin, or any other recognition protein. The ability to turn on or off biological recognition processes on a molecular level should lead to many interesting, diverse and unique applications. In this proposal we focus on the affinity recognition process, and its applications in affinity separations, diagnostics and biosensors.

在这个提案中，我们建议结合（a）最新的蛋白质工程 使用(b)刺激响应性聚合物组合物生产(c)的技术 响应性聚合物工程蛋白 (RP-EP) 缀合物具有独特的 行为和用途。 更具体地说，我们建议共轭 通过其末端之一对温度、pH 或光敏感的低聚物 终止于活性识别位点附近或之内的特定位点 选择蛋白质，然后施加温度、pH 或光刺激 tern "（即允许绑定）或"off"（即减少绑定 亲和力，完全阻止结合，或弹出先前结合的受体） 目标配体或细胞与蛋白质识别的结合 地点。 该蛋白质可以是抗体、酶、激素、整合素、 白蛋白、链霉亲和素或任何其他识别蛋白。 该蛋白质可以 可以是抗体、酶、激素、整联蛋白、白蛋白、链霉亲和素， 或任何其他识别蛋白。 打开或关闭生物的能力 分子水平上的识别过程应该会导致许多有趣的、 多样化且独特的应用。 在这个提案中，我们重点关注亲和力 识别过程及其在亲和分离中的应用， 诊断和生物传感器。