14-3-3 PROTEIN FUNCTION IN CELL GROWTH AND MOTILITY

14-3-3 细胞生长和运动中的蛋白质功能

• 批准号: 2023493
• 负责人: ANTHONY JUSTIN MUSLIN
• 金额: $ 15.2万
• 依托单位: BARNES-JEWISH HOSPITAL
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-05-01 至 2002-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2023493
• 关键词: 3T3 cells; SDS polyacrylamide gel electrophoresis; Xenopus oocyte; actins; autoradiography; binding proteins; biological signal transduction; cell growth regulation; cell motility; enzyme activity; fluorescence microscopy; high performance liquid chromatography; immunoprecipitation; in situ hybridization; microinjections; oligonucleotides; phosphoserine; polymerase chain reaction; polymerization; protein kinase; protein signal sequence; protein structure function; radionuclides; tissue /cell culture; yeast two hybrid system

DESCRIPTION: This proposal centers on understanding the function of the 14-3-3 signaling molecule. The major goal is to establish that 14-3-3 specifically binds to serine-phosphorylated proteins and helps to assemble signaling complexes that regulate cell proliferation, differentiation, and cell motility. This hypothesis will be tested through three specific experimental aims. The first will be to assess the ability of 14-3-3 to bind to specific phosphoserine motifs in vivo (through the use of mutant forms of the Raf-1 kinase). The second aim focuses on examining the role of 14-3-3 in Raf-1-mediated signal transduction through an analysis of the effects of specific 14-3-3 inhibitors on Xenopus laevis embryo development. The third aim will then center on examining the role of 14-3-3 in the regulation of acting polymerization and cell motility in cultured fibroblasts. The expectation is that the results of these experiments will establish the importance of the 14-3-3 signaling molecules in cell growth regulation and development.

描述：本提案的重点是理解 14-3-3信号分子。 主要目标是建立 14-3-3 特异性结合丝氨酸磷酸化蛋白并帮助组装 信号复合物调节细胞增殖、分化和 细胞运动。 这个假设将通过三个具体的测试来检验 实验目的。 首先是评估 14-3-3 的能力 结合体内特定的磷酸丝氨酸基序（通过使用突变体 Raf-1 激酶的形式）。 第二个目标侧重于审查 通过分析 Raf-1 介导的信号转导中的 14-3-3 特异性 14-3-3 抑制剂对非洲爪蟾胚胎发育的影响。 第三个目标将集中于研究 14-3-3 在比赛中的作用。 培养物中作用聚合和细胞运动的调节 成纤维细胞。 期望这些实验的结果将 确定 14-3-3 信号分子在细胞生长中的重要性 监管和发展。