RABPHILIN3A AND EXOCYTOSIS AND CHROMAFFIN CELLS

RABPHILIN3A 与胞吐作用和嗜铬细胞

• 批准号: 2017024
• 负责人: RONALD W HOLZ
• 金额: $ 23.71万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-05-14 至 2001-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2017024
• 关键词: antisense nucleic acid; chemical kinetics; chromaffin cells; cytoskeleton; exocytosis; mutant; protein structure function; proteins; secretion; single cell analysis; somatotropin; tissue /cell culture; transfection

DESCRIPTION: Exocytotic release of hormones and neurotransmitters is central to intercellular communication and synaptic transmission and is fundamental to the normal function of the endocrine, cardiovascular and nervous systems. The long-term goal of the application is to understand the biochemical and physiological mechanisms underlying regulated exocytosis. Specifically, this application will elucidate the role of rabphilin3a in the pathway. Rab GTPases are involved in vesicular trafficking in the secretory and endocytotic pathways. Rabphilin3a was identified in brain as a possible effector for rab3a. The investigators recently found that rabphilin3a is expressed with rab3a in adrenal chromaffin cells and enhances calcium dependent secretion. The focus of this proposal is to investigate the mechanisms by which rabphilin3a functions in regulated exocytosis. The proposal will: 1) elucidate the mechanism by which rabphilin3a enhances exocytosis in bovine adrenal chromaffin cells using both biochemical and electrophysiological measures of secretion. 2) Determine the relationship between subcellular localization of rabphilin3a and mutants and function in secretion. 3) Investigate possible new members of the rabphilin3a pathway including adducin. A variety of techniques will be used to study rabphilin3a function in chromaffin cells including transient-expression approach for biochemical studies of secretion and electrophysiological/microamperometric techniques from single cell studies.

描述：激素和神经递质的胞吐释放是 细胞间通信和突触传播的中心，IS 对于内分泌，心血管和 神经系统。 该应用程序的长期目标是了解 受调节胞吐作用的基础生化和生理机制。 具体而言，此应用将阐明Rabphilin3a在 路径。 Rab GTPases参与分泌的囊泡贩运 和内吞途径。 Rabphilin3a在大脑中被鉴定为 Rab3a的效应器。 调查人员最近发现Rabphilin3a是 用Rab3a在肾上腺染色体细胞中表达并增强钙 依赖分泌。 该提议的重点是调查 rabphilin3a在调节胞吐作用中起作用的机制。 这 提案将：1）阐明Rabphilin3a增强的机制 使用生化和 分泌的电生理测度。 2）确定关系 在Rabphilin3a的亚细胞定位与突变体之间以及在功能 分泌。 3）调查Rabphilin3a途径的新成员 包括添加剂。 各种技术将用于学习 Rabphilin3a在铬蛋白细胞中的功能，包括瞬态表达 分泌生化研究的方法 来自单细胞研究的电生理/微观测量技术。