CLATHRIN LIGHT CHAIN IN YEAST

酵母中的网格蛋白轻链

基本信息

批准号：
2391795
负责人：
KRISTEN M HUANG
金额：
$ 2.86万
依托单位：
CASE WESTERN RESERVE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1997
资助国家：
美国
起止时间：
1997-04-01 至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/2391795
关键词：
Saccharomyces cerevisiae clathrin intermolecular interaction protein structure function

项目摘要

In eukaryotes, movement of material into and through the cell is mediated by the budding, transport and fusion of membrane vesicles. Some of these vesicles are surrounded by coat proteins which are thought to promote vesicle formation. Clathrin-coated vesicles are required for the uptake of important materials such as nutrients, growth hormones, cholesterol and viruses and clathrin function is required for the normal growth and development of all eukaryotic organisms. The absence of clathrin can lead to aberrant growth of even cell death. Clathrin coat are composed of clathrin triskelions and each clathrin triskelion is composed of three clathrin heavy chains (HC's) and three non-covalently associated clathrin light chains (LC's). The HC's provide the structural backbone of the clathrin lattice but the role of the LC's in clathrin function remains unclear. This research proposal will examine the function of clathrin LC's in Saccharomyces cerevisiae. There is preliminary evidence that LC's stabilize HC's. This will be investigated further by examining the HC's in cells where the clathrin LC gene has been disrupted. The region of the LC important for biological function will also be explored by examining strains that express truncated and deleted forms of the LC. Also, the regions of the HC required for interaction with the LC will be delineated using the powerful two-hybrid system. The two-hybrid system will also be used to identify proteins that interact with and possibly regulate the LC's. The identification of proteins that bind to LC's may help elucidate the proposed regulatory role of LC's. The research project will provide valuable information about the role of LC's in the function of clathrin.

在真核生物中，材料进入和通过细胞的运动是介导的膜囊泡的萌芽，运输和融合。其中一些囊泡被认为可以促进的外套蛋白包围囊泡形成。摄取需要网格蛋白涂层的囊泡重要的材料，例如营养，生长激素，胆固醇正常生长需要病毒和网格蛋白功能所有真核生物的发展。缺乏网状蛋白可以导致甚至细胞死亡的异常生长。网状蛋白外套组成网格蛋白的triskelions和每个网状蛋白Triskelion均由三个组成网格蛋白重链（HC）和三个无共价关联的网格蛋白轻链（LC）。 HC提供了结构性的骨干网格蛋白晶格，但LC在网格蛋白功能中的作用仍然存在不清楚。该研究建议将检查网格蛋白的功能 LC在酿酒酵母中。有初步证据表明 LC的稳定HC。这将通过检查 HC在网细胞中破坏的细胞中。该地区 LC对生物功能的重要重要性也将由检查表达LC的截断和删除形式的菌株。同样，与LC相互作用所需的HC区域将是使用功能强大的两杂交系统进行描述。两个杂交系统还将用于识别与并可能相互作用的蛋白质调节LC的。鉴定与LC可能结合的蛋白质有助于阐明LC的拟议监管作用。研究项目将提供有关LC在网格蛋白的功能。