MYCOPLASMAS AND THE RHEUMATIC DISEASES

支原体和风湿性疾病

• 批准号: 2081873
• 负责人: KIM S. WISE
• 金额: $ 20.19万
• 依托单位: UNIVERSITY OF MISSOURI-COLUMBIA
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-03-01 至 1998-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2081873
• 关键词: B lymphocyte; Mycoplasma; connective tissue disorder diagnosis; host organism interaction; human subject; humoral immunity; laboratory mouse; membrane lipids; membrane proteins; microorganism classification; molecular pathology; nonblood lipoprotein; pathologic process; polymerase chain reaction; rheumatism; rheumatoid arthritis; serology /serodiagnosis; transposon /insertion element

There is a rationale for further evaluating the role of mycoplasmas in the human rheumatic diseases; this rationale is based on (i) the established role of mycoplasmas as etiological agents of several animal arthritides; (ii) accumulating evidence associating the human mycoplasmal flora with aspects of inflammatory rheumatic diseases; and (iii) recent evidence describing mutational systems driving adaptive surface variation of human mycoplasma species. This project focuses on characterizing the interaction of human mycoplasmas with their host, with specific emphasis on individuals with defined rheumatic disorders. To further delineate a possible role of mycoplasmas in these diseases, it is specifically proposed to: 1) examine disease sites by selective PCR to detect, classify, and characterize mycoplasmas associated with pathologic lesions; 2) characterize a newly identified and highly individual B cell response to select subsets of epitope repertoires displayed on variant surface membrane lipoproteins of human mycoplasmas; and 3) define the molecular basis of surface lipoprotein variation in these mycoplasma species. Techniques include PCR amplification and analysis of target sequences in host-derived materials, immunologic screening protocols to define patterns of serologic reactivities, and characterization of membrane protein genes by transposon mutagenesis and standard molecular techniques. It is anticipated that information emerging from these studies will directly contribute to an understanding of the nature of mycoplasma infections, their association with the rheumatic diseases, the mechanism of pathogenesis, methods for detection, and formulation of strategies to control these chronic disorders.

有理由进一步评估支原体在 人类风湿病；这一理由基于 (i) 既定的 支原体作为几种动物关节炎病原体的作用； (ii) 积累人类支原体菌群与 炎症性风湿性疾病的各个方面； (iii) 最近的证据 描述驱动人类适应性表面变化的突变系统 支原体种类。该项目重点关注交互的特征 人类支原体及其宿主的研究，特别强调 患有明确风湿性疾病的个体。为了进一步划定一个 支原体在这些疾病中可能发挥的作用，特别是 建议：1）通过选择性 PCR 检查疾病部位来检测， 对与病理病变相关的支原体进行分类和表征； 2) 表征新鉴定的高度个体化的 B 细胞反应 选择变体表面上显示的表位库子集 人支原体膜脂蛋白； 3）定义分子 这些支原体物种表面脂蛋白变异的基础。 技术包括 PCR 扩增和靶序列分析 宿主衍生材料、定义模式的免疫筛选方案 血清学反应性和膜蛋白基因的表征 通过转座子诱变和标准分子技术。这是 预计这些研究中出现的信息将直接 有助于了解支原体感染的性质， 它们与风湿性疾病的关系、机制 发病机制、检测方法和制定策略 控制这些慢性疾病。