52KD RO/SSA AUTOANTIGEN

52KD RO/SSA 自身抗原

• 批准号: 2517467
• 负责人: Mark Frank
• 金额: $ 14.82万
• 依托单位: OKLAHOMA MEDICAL RESEARCH FOUNDATION
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-09-30 至 1999-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2517467
• 关键词: DNA binding protein; autoantibody; autoantigens; clinical research; genetic polymorphism; genetic promoter element; human subject; molecular pathology; nucleic acid sequence; systemic lupus erythematosus

DESCRIPTION (Adapted from Investigator's abstract): Autoantibodies to a 52kd Ro/SSA protein occur in some patients with SLE, and are inversely correlated with renal disease in Black American SLE patients. A polymorphism of the 52kd Ro gene correlates with this finding, leading to the hypotheses in this project that, first, this polymorphism may be a genetic marker for an allelic variant of 52kd Ro which preferentially occurs in lupus patients, or secondly, that this polymorphism is a marker for a mutation which disregulates the expression of this gene. Sequence analysis and functional promoter studies will be used in Aim 1 to address these hypotheses. In Aim 2, the DNA binding properties of the 52kd Ro protein will be investigated and the possible role of this protein influencing gene expression will be studied. Aim 3 will quantitate antibodies to 52kd Ro in subsets of lupus patients and Aim 4 will study the pathogenicity of these autoantibodies focusing on lesions of cutaneous lupus and binding of these autoantibodies to kerotinocytes after UV irradiation, a trigger for the photosensitive reactions of cutaneous lupus patients.

描述（根据研究者的摘要进行了改编）：自动抗体 52KD RO/SSA蛋白发生在某些SLE患者中，并且是成反比的 与黑人SLE患者的肾脏疾病有关。 一个 52KD RO基因的多态性与这一发现相关，导致 该项目中的假设，首先，这种多态性可能是 52KD RO的等位基因变体的遗传标记，优先发生 在狼疮患者中，或第二，这种多态性是 突变无视该基因的表达。 序列分析 AIM 1将使用功能启动子研究来解决这些问题 假设。 在AIM 2中，52KD RO蛋白的DNA结合特性 将研究该蛋白质影响基因的可能作用 表达将被研究。 AIM 3将将抗体定量为52KD RO 狼疮患者和AIM 4的子集将研究这些的致病性 自身抗体，重点是皮肤狼疮病变和这些结合 紫外线照射后自身抗体对角蛋白形成细胞，这是触发的 皮肤狼疮患者的光敏反应。