STRUCTURE AND FUNCTION OF HUMAN SALIVARY MUCINS

人唾液粘蛋白​​的结构和功能

• 批准号: 2430132
• 负责人: ROBERT F TROXLER
• 金额: $ 19.93万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-08-01 至 1999-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2430132
• 关键词: SDS polyacrylamide gel electrophoresis; chromosome translocation; complementary DNA; gene expression; genetic library; genetic polymorphism; human genetic material tag; human tissue; immunocytochemistry; in situ hybridization; molecular cloning; molecular weight; monoclonal antibody; mucins; northern blottings; nucleic acid repetitive sequence; nucleotides; protein purification; protein sequence; protein structure function; saliva; sublingual gland; submandibular gland; western blottings

DESCRIPTION (Adapted from the applicant's Abstract): High molecular weight salivary mucins (MG1s) are multimeric glycoconjugates secreted by specialized acinar cells in submandibular, sublingual and minor salivary glands. In the oral cavity, mucins protect oral surfaces against desiccation, form a selectively permeable diffusion barrier between underlying surfaces and the external environment, lubricate hard and soft tissues to minimize mechanical injury, facilitate speech and swallowing, and provide a physical barrier to protect against colonization of potentially harmful microbes and viruses. Mucins have been difficult to isolate and characterize because of their large size, polymeric state and high oligosaccharide content. No information is available on the structural organization or primary structure of high molecular weight salivary mucins although there is mounting evidence that unmistakably points to existence of distinct mucins in human submandibular and sublingual glands. Recently, the Principal Investigator has isolated and sequenced clones encoding portions of a high molecular weight mucin (HSLM) from human sublingual gland, and have found that the deduced amino acid sequences can be aligned with the cysteine-rich carboxy-terminal region of human intestinal mucin MUC2, porcine submaxillary gland mucin, and bovine submaxillary gland mucin. The specific aims are to: 1. Characterize the structural organization of HSLM by determining the nucleotide and deduced amino acid sequences of the tandem repeat region, and the N-terminal and C-terminal regions flanking the repeats, the tissue distribution of transcripts, the chromosomal localization of the HSLM gene and the occurrence of HSLM homologues in other vertebrates. 2. Identify the high molecular weight mucin (HSMM) in submandibular gland by isolating clones for this mucin from a human submandibular gland cDNA library, determining the nucleotide and deduced amino acid sequences of the coding region, and examining the expression patterns, gene localization and evolutionary relationships of this mucin.

描述（改编自申请人的摘要）：高分子量 唾液粘蛋白​​（MG1）是由多聚体糖缀合物分泌的 下颌，舌下和小唾液中的专门腺泡细胞 腺体。 在口腔中，粘蛋白保护口腔表面免受 干燥，形成有选择性渗透的扩散屏障 底层表面和外部环境，硬润滑 组织以最大程度地减少机械损伤，促进言语和吞咽，并且 提供物理障碍以防止潜在的殖民 有害的微生物和病毒。 粘蛋白很难分离，并且 特征是因为它们的尺寸较大，聚合物状态和高 寡糖含量。 没有有关结构的信息 高分子量唾液粘蛋白​​的组织或主要结构 尽管有越来越多的证据表明 人类下颌和舌下腺中的独特粘蛋白。 最近， 主要研究者已经隔离和测序编码部分的克隆 来自人类舌下腺的高分子量粘蛋白（HSLM）， 已经发现推导的氨基酸序列可以与 人肠粘膜MUC2的富含半胱氨酸的羧基末端区域， 猪超X.粘膜粘蛋白和牛超X.粘膜粘蛋白。 这 具体目的是：1。表征HSLM的结构组织 通过确定串联的核苷酸和推导的氨基酸序列 重复区域以及N末端和C末端区域的侧面 重复，转录本的组织分布，染色体 HSLM基因的定位和其他HSLM同源物在其他 脊椎动物。 2。识别高分子量粘蛋白（HSMM） 下颌腺通过与人类的粘蛋白分离的克隆来分离 下颌下腺cDNA库，确定核苷酸并推导 编码区域的氨基酸序列，并检查表达 该粘蛋白的模式，基因定位和进化关系。