REGULATION OF ACINAR CELL PROLIFERATION

腺泡细胞增殖的调节

• 批准号: 2389418
• 负责人: MICHAEL G HUMPHREYS-BEHER
• 金额: $ 19.12万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-08-01 至 2000-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2389418
• 关键词: acinar cell; active sites; antisense nucleic acid; calmodulin dependent protein kinase; cell growth regulation; cell proliferation; enzyme mechanism; epidermal growth factor; galactosyltransferases; gene expression; genetic library; genetic regulatory element; isoproterenol; laboratory mouse; laboratory rat; molecular cloning; parotid gland; protein tyrosine kinase; reporter genes; site directed mutagenesis; transcription factor; transfection

DESCRIPTION (Adapted from the investigator's Abstract): The present proposal continues the ongoing efforts of this investigator to elucidate the biological effects of beta 1,4-galactosyltransferase (Gal Tase), and the expression of GTA-K on acinar cell growth response. The following specific aims have been proposed: (1) Elucidate the chromosomal structure of the GTA-K and determine the role of the regulatory sequences in the expression of this protein. (2) To study the biological effects of the GTA-K through site-directed mutagenesis of various functional domains. It is anticipated that these studies will provide new information about one possible mechanism involved in the regulation of acinar cell growth. A model which has been widely used, for almost three decades, to study acinar cell growth is the acinar cell proliferation in response to beta adrenergic stimulation. This study is aimed towards further characterizing the mechanisms associated with this proliferative event.

描述（改编自调查员的摘要）：现在 提案继续该调查员正在进行的努力阐明 β1,4-半乳糖基转移酶（GAL Tase）和 GTA-K在腺泡细胞生长反应上的表达。 以下特定 已经提出了目的：（1）阐明 GTA-K并确定调节序列在表达中的作用 该蛋白质。 （2）研究GTA-K的生物学作用 各种功能域的位置定向诱变。 预计 这些研究将提供有关一种可能机制的新信息 参与调节腺泡细胞生长。 一个模型 在近三十年中，广泛使用用于研究腺泡细胞生长的是 响应β肾上腺素能刺激的腺泡细胞增殖。 这 研究旨在进一步表征与 这个扩散事件。