PREPARATION OF GONADAL CELLS AND CELL FRACTIONS

性腺细胞和细胞组分的制备

• 批准号: 2316064
• 负责人: NORMAN N BEAUDRY
• 金额: $ 34.54万
• 依托单位: COVANCE LABORATORIES, INC.
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-12-11 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2316064
• 关键词: PREPARATION; GONADAL; CELLS; CELL; FRACTIONS

The program on analysis of gonadotropin receptors and actions of gonadotropic hormones is an important component of the Endocrinology and Reproduction Research Branch scientific research objectives. This program is supported by a Contract that provides large amounts of well characterized preparations including purified gonadal cells, various short term and established cell cultures, gonadal tissues and cell fractions, and receptor preparations. Gonadal cells are prepared acutely from a large number of control animals and under multiple protocols. The contract supports work in reproductive biology allowing investigators to perform studies on the binding characteristics of gonadotropin and prolactin receptors of testicular, ovarian, Nb2 lymphoma and several transfected cells, and cell membranes; and of the biological characterization of LH and related hormones, and studies on the mechanisms of hormone-receptor interaction, signal transduction, interaction between signalling pathways, and modulation of key metabolic steps of the androgen biosynthetic pathways.

分析促性腺激素受体和动作的计划 促性腺激素激素是内分泌学和 繁殖研究部科学研究目标。 这个程序 由提供大量井的合同支持 表征包括纯化的性腺细胞的制剂，各种短 术语和已建立的细胞培养物，性腺组织和细胞部分， 和受体制剂。 性腺细胞是从A急性制备的 大量对照动物和多种方案。 这 合同支持生殖生物学的工作，使研究人员能够 对促性腺激素和 睾丸，卵巢，NB2淋巴瘤的催乳素受体和几种 转染的细胞和细胞膜；和生物学 LH和相关激素的表征，以及有关机制的研究 激素受体相互作用，信号转导，相互作用 信号通路和雄激素的关键代谢步骤的调节 生物合成途径。