ATHEROGENIC EFFECTS OF DIETARY OIL AND NUTRITION IN A RAT MODEL

膳食油和营养对大鼠模型的动脉粥样硬化效应

• 批准号: 3734102
• 负责人: KOTHAPA CHETTY
• 金额: --
• 依托单位: GRAMBLING STATE UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3734102
• 关键词: HMG coA reductases; atherosclerosis; blood lipid; cardiovascular disorder epidemiology; cholanate compound; cholesterol; dietary lipid; disease /disorder model; electron microscopy; feces analysis; heart; histopathology; laboratory rat; light microscopy; lipid metabolism; liver; low density lipoprotein; minority institution research support; nutrition related tag; sterols; vegetable oils; very low density lipoprotein

The long-term goal of the current project is to understand the mechanisms by which different dietary oils, cholesterol and nutrition interact on the atherogenic effects in rats. Our specific aims are to determine how feeding the different oils influence the cholesterol levels in a normal diet and the cholesterol added diet. Male Sprague-Dawley rats will be fed various purified diets with either corn oil, olive oil or canola oil with and without cholesterol for a short period or long period. The samples of serum, heart and liver tissues will be obtained and analyzed for the levels of lipids, total cholesterol, HDL and LDL cholesterol bile acids and fecal neutral sterols. Also, the enzymatic assays for HMg CO-A reductase, and cholesterol 7-alpha hydroxylase will be observed to determine the effects of dietary oils on cholesterol metabolism. Also, the structural and ultrastructural changes of heart and liver tissues due to the influence of diets will be studied using light microscope and electron microscope. The students will be trained in the basic cardiovascular research and nutrition and prepare to present papers in scientific meetings and publish papers in scientific journals.

当前项目的长期目标是了解其机制 不同的膳食油、胆固醇和营养物质通过这些相互作用对 对大鼠的动脉粥样硬化影响。 我们的具体目标是确定如何 喂养不同的油会影响正常人的胆固醇水平 饮食和添加胆固醇的饮食。 雄性斯普拉格-道利大鼠将被喂养 各种含有玉米油、橄榄油或菜籽油的纯化饮食 并且短期或长期不含胆固醇。 的样本为 将获取并分析血清、心脏和肝脏组织 脂质、总胆固醇、HDL 和 LDL 胆固醇胆汁酸水平 和粪便中性甾醇。 此外，HMg CO-A 的酶测定 还原酶和胆固醇 7-α 羟化酶将被观察到 确定膳食油对胆固醇代谢的影响。 还， 心脏和肝脏组织的结构和超微结构变化 将使用光学显微镜研究饮食的影响 电子显微镜。 学生将接受基础培训 心血管研究和营养并准备在以下领域发表论文 科学会议并在科学期刊上发表论文。