PHOSPHORIMAGER SYSTEM

磷光成像系统

• 批准号: 2284259
• 负责人: ALBERT J COUREY
• 金额: $ 8.98万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-04-01 至 1996-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2284259
• 关键词: Animalia; biomedical equipment purchase; computer graphics /printing; computer program /software; molecular dynamics; phosphorus; radionuclide imaging /scanning

This application requests funds for the purchase of a phosphorimager for UCLA. This instrument is designed to replace conventional X-ray film-based autoradiography for imaging, analyzing and quantitating beta-radiation. The source of radiation is placed in contact with an imaging screen to form a latent image on the screen. The imaging screen is "developed" after an appropriate exposure time by a laser scanner which "releases" the image in the form of photons. Information from the photon detector is stored digitally where it is ready for quantitative analysis or display. The advantages of the phosphorimager over traditional methods are several: 1) increased sensitivity by a factor of 10-200 over traditional methods, 2) a linear response range for data collection that is greater than that for X-ray film by a factor of 10-3 - 10-4, and 3) data storing and processing capabilities that make it possible to objectively quantify signals over the dynamic range of the instrument from a single exposure. These features make previously impractical experiments "doable", they allow the experimenter to extract quantitative information from the stored data and they make it possible to normalize and compare multiple samples. The research descriptions of the major users (Courey - PI - Transcriptional Control of Pattern Formation in the Drosophila Embryo, Berk - Regulation of Transcription by Adenovirus, Clarke - Control of Eukaryotic Membrane Function by Methylation, Gober - Generation of Asymmetry During Caulobacter Development, Gralla - Mechanism of Transcriptional Initiation, Gunsalus - Regulation of Cellular Respiratory Functions, Johnson - Mechanism of site-specific DNA recombination and growth phase regulation by Fis, Merchant - Metalloprotein Biosynthesis in Chlamydomonas, Miller - Invasion of epithelial cells by Yersinia and Salmonella, Morrison - Regulation of Antibody Biosynthesis and Function, Sigman -Chemical Nucleases as Probes of Nucleic Acid Structure-Function Relationships, Simons - Translational Control and RNA Structure, Tobin - Phytochrome Regulation of Specific Gene Expression, Valentine -Functional Studies of Cytosolic Anti-Oxidant Proteins, Weiss - Metabolic Organization in Neurospora) justify the use of the instrument to enhance their research capabilities on each of their NIH-funded research projects. A plan for makihg the instrument available to the large community of researchers in the biomedical sciences at UCLA is in place and resources for its long-term operation and maintenance are guaranteed. The technology is well-developed and has been employed in basic biomedical research for many years. The users include a large group of active investigators, all of whom are engaged in research on health-related, NIH-funded projects representing studies on fundamental aspects of biochemistry, molecular, cell and developmental biology. By enhancing the effectiveness of the research efforts on these individual projects, this application benefits and su orts health-related research.

本申请要求购买资金 加州大学洛杉矶分校的磷光受益剂。该乐器旨在更换 传统的基于X射线膜的放射自显影，用于成像， 分析和定量β-辐射。辐射的来源 与成像屏幕接触以形成潜在图像 在屏幕上。成像屏幕是“开发”的 通过“释放”的激光扫描仪的适当暴露时间 图像以光子的形式。来自光子检测器的信息 以数字方式存储在准备定量分析或 展示。磷象比传统的优势 方法有几种：1）提高灵敏度的倍数为10-200倍 在传统方法上，2）数据的线性响应范围 比X射线膜更大的收集因素 10-3-10-4和3）数据存储和处理功能， 使能够客观地量化动态信号 仪器的范围来自一次曝光。这些功能构成 以前不切实际的实验“可行”，它们允许 实验者从存储的 数据，它们使得可以正常化和比较多个 样品。主要用户的研究描述（Courey -Pi - 果蝇中模式形成的转录控制 Berk Embryo-腺病毒对转录的调节 - 克拉克 - 通过甲基化控制真核膜功能，Gober- 在 花椰菜发育，格拉拉 - 转录机理 启动，枪 - 调节细胞呼吸道 功能，约翰逊 - 位点特异性DNA重组的机制 FIS，商人 - 金属蛋白的生长阶段调节 衣原体中的生物合成，米勒 - 上皮的入侵 细胞由耶尔森氏菌和沙门氏菌，莫里森 - 抗体调节 生物合成和功能，西格曼化学核酸酶作为探针 核酸结构功能关系，西蒙斯 - 翻译控制和RNA结构，托宾 - 植物色素 调节特定基因表达，情人节功能 胞质抗氧化蛋白的研究，Weiss-代谢 在Neurospora的组织）证明使用该工具的合理性 增强他们在NIH资助的每个资金的研究能力 研究项目。 Makihg的计划 生物医学科学研究人员的大型研究人员 加州大学洛杉矶分校的长期运营和资源 保证维护。该技术已发达，并且 多年来已在基本的生物医学研究中使用。这 用户包括一大批活跃的调查员 从事与健康相关的NIH资助项目的研究 代表有关生物化学基本方面的研究， 分子，细胞和发育生物学。通过增强 研究工作对这些单个项目的有效性， 该应用程序受益于与健康相关的研究。