DEVELOPMENT OF NEW SCREENING TESTS FOR HTLV-III

HTLV-III 新筛查测试的开发

• 批准号: 2312847
• 负责人: ROBERT H SINGER
• 金额: $ 40.2万
• 依托单位: UNIV OF MASSACHUSETTS MED SCH WORCESTER
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-09-30 至 1994-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2312847
• 关键词: DEVELOPMENT; NEW; SCREENING; TESTS; HTLV

This proposal focuses on the development of non-radioisotopic in situ hybridization assay to detect LAV/HTLV-III infected cells, and to develop a method for recovery of AIDS virus from infected sources using a T4+ cell line that expresses the transactivating translational gene (tat) of LAV/HTLV-III as the basis of a virus detection/culture system. The offeror emphasize that in situ hybridization offers advantages over immunologic methods of detecting virus infected cells since it is able to detect infected cells which are not expressing viral proteins. Their method for in situ hybridization uses non-isotopic detection which allows easy visualization of infected cells by standard microscopy within one working day. The objectives of the current proposal are to simplify the method so that it can be adapted for clinical use, and to improve the detection to the point where a single copy of virus DNA or RNA can be detected.

该提案重点是原位非降量异位的发展 杂交分析以检测LAV/HTLV-III感染细胞，并发展 使用T4+细胞从受感染来源中恢复艾滋病病毒的方法 表达反式激活翻译基因（TAT）的线 LAV/HTLV-III作为病毒检测/培养系统的基础。 要约人 强调原位杂交比免疫学具有优势 检测病毒感染细胞的方法，因为它能够检测 未表达病毒蛋白的感染细胞。 他们的方法 原位杂交使用非同位素检测，可以轻松 通过标准显微镜在一个工作中通过标准显微镜可视化感染细胞 天。 当前建议的目标是简化该方法，因此 它可以适应临床使用，并将检测提高到 可以检测到病毒DNA或RNA的单个副本的点。