RODENT FOUNDATION COLONIES

啮齿动物基金会殖民地

• 批准号: 2311656
• 负责人: JAMES D RUSSELL
• 金额: $ 10.76万
• 依托单位: SIMONSEN LABORATORIES
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-09-14 至 1994-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2311656
• 关键词: RODENT; FOUNDATION; COLONIES

The purpose of this project is to operate, maintain and provide pedigreed, defined microflora associated rodent foundation colonies for supplying breeders to the expansion colonies of the rodent production centers. Offspring from genetically defined inbred pedigreed stock obtained from NIH Repository or other sources will be Cesarian derived, maintained in isolators and associated with defined microflora. Offspring from these foundation stock will be used to supply breeders to the expansion colonies of new rodent production colonies at a rate of 30 breeding pairs of Fischer 344/N rats and 45 breeding pairs of C57BL/6N and C3H/HeN MTV- mice and appropriate number of breeding pairs of other strains per month. Fishcer 344/N rats and B6C3F1 hybrid mice are the selected experimental animal species for evaluation of chemicals for toxicologic and carcinogenic properties under DTRT/NTP. The genetic integrity of the apparent generations of Fischer 344/N rats and the C57BL/6N and C3H/HeN MTV- mice to produce the B6C3F1 hybrid is essential for developing reliable and accurate research data in animal experiments. Centralized foundation colonies will provide better control on genetic integrity of all the breeders in the expansion and production colonies of rodent production centers.

该项目的目的是操作，维护和提供 造型，定义的微生物相关的啮齿动物基础菌落 为啮齿动物生产的膨胀菌落提供育种者 中心。 从遗传定义的近孕期库存的后代获得的后代 NIH存储库或其他来源将是核心的，维持在 隔离器并与定义的菌群相关。这些后代 基础库存将用于向育种者提供扩展 新啮齿动物生产菌落的菌落以30对 Fischer 344/N大鼠和45对C57BL/6N和C3H/HEN MTV-的繁殖对 小鼠和每月其他菌株的适当数量的繁殖对。 渔夫344/N大鼠和B6C3F1杂交小鼠是选定的实验 用于评估毒理学化学物质的动物物种 DTRT/NTP下的致癌特性。遗传完整 Fischer 344/N大鼠的明显世代和C57BL/6N和C3H/HEN 生产B6C3F1混合动力的mtv-ciels对于发展至关重要 动物实验中可靠，准确的研究数据。集中 基础殖民地将更好地控制 啮齿动物的扩张和生产菌落中的所有育种者 生产中心。