Core C: IgSeq and NGS Core

核心 C：IgSeq 和 NGS 核心

基本信息

批准号：
10549607
负责人：
Jiwon Lee
金额：
$ 36.36万
依托单位：
BOSTON CHILDREN'S HOSPITAL
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-08-01 至 2028-03-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10549607
关键词：
Antibodies Antibody Repertoire Antibody Response Antigens Attention B cell repertoire B-Lymphocytes B-cell receptor repertoire sequencing DNA Sequencing Facility Data Data Analyses Development Elderly Engineering Exposure to Funding Goals Hemagglutinin Human Humoral Immunities Immune Individual Infant Infection Influenza Influenza Hemagglutinin Influenza vaccination Infrastructure Investigation Length Liquid Chromatography Mediating Memory B-Lymphocyte Molecular Monitor Monoclonal Antibodies Neuraminidase Online Systems Plasmablast Play Population Proteomics Recombinants Research Personnel Research Support Resolution Resources Role Sampling Seasons Sequence Analysis Series Serum Services Shapes Structure Technology Time Transcript Vaccination Vaccines Viral Virus Diseases Work age group analytical tool college data repository database query design imprint influenza virus vaccine next generation sequencing professor programs response seasonal influenza success tandem mass spectrometry tool transcriptomics vaccination strategy young adult

项目摘要

ABSTRACT A principal goal of the Program Project is to chart the changes in influenza antibody repertoire following vaccination or infection and to build on those results to direct immunogen design efforts. Core C, the Ig-Seq and NGS Core, will serve the overall goals of the Program Project by providing high-throughput antibody repertoire profiling services. Specifically, it will provide in-depth NextGen Sequencing (NGS) of antibody transcripts from B cells (BCR-Seq) and quantitative high-resolution proteomic analysis of serum antibody molecules (Ig-Seq). From these sequence analyses, the Core will generate full-length antibody sequences for recombinant expression and characterizations as monoclonal antibodies.The Core will also serve all Projects and Cores of the Program Project by providing a centralized data repository, management of all antibody sequence data, robust and consistent data analysis. The specific aims of Core C are: (1) provide the NGS service to define the B cell repertoire; (2) provide the proteomic-based antibody analysis service to define serum antibody repertoire; (3) provide a centralized data repository and analysis for antibody sequences generated by all the Projects and Cores; and (4) expand and optimize the provided services. The Ig-Seq and NGS Core is critical for the success of the Program Project, offering high-throughput tools for deep and comprehensive monitoring of changes in three distinct components of antibody repertoires: serum antibody molecules, memory B cells, and plasmablasts.

抽象的该计划项目的主要目标是绘制以下流感抗体库的变化图：疫苗接种或感染，并根据这些结果指导免疫原设计工作。核心 C，Ig-Seq 和NGS Core，将通过提供高通量抗体服务于该计划项目的总体目标曲目分析服务。具体来说，它将提供抗体的深度下一代测序（NGS） B 细胞转录本 (BCR-Seq) 和血清抗体的定量高分辨率蛋白质组分析分子（Ig-Seq）。根据这些序列分析，Core 将生成全长抗体序列作为单克隆抗体的重组表达和表征。核心还将服务于所有项目和项目的核心通过提供集中的数据存储库，管理所有抗体序列数据、稳健且一致的数据分析。 Core C的具体目标是：（1）提供NGS 定义 B 细胞库的服务； (2) 提供基于蛋白质组学的抗体分析服务来定义血清抗体库； (3) 提供集中的抗体序列数据存储和分析由所有项目和核心生成；（四）拓展和优化提供的服务。 Ig-Seq 和 NGS Core 对于该计划的成功至关重要，它为深度和广度的研究提供高通量工具。全面监测抗体库三个不同成分的变化：血清抗体分子、记忆 B 细胞和浆母细胞。