MAINTENANCE AND DEVELOPMENT OF INBRED AND CONGENIC RES

近交系和同源系的维护和开发

• 批准号: 3606048
• 负责人: DANIELLA LIVNAT
• 金额: $ 65.55万
• 依托单位: HAZLETON GOVERNMENT SERVICES DIVISION
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-02-01 至 1993-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3606048
• 关键词: animal colony; antiserum; complementary DNA; genetic strain; genetically modified animals; hybridomas; inbreeding; laboratory mouse; skin transplantation

The objectives of this contract are: 1) to maintain a colony of approximately 60 strains of inbred mice by strict pedigreed brother-sister matings; 2) to breed and develop new congenic mouse strains; 3) to make selective crosses and backcrosses between these strains; 4) to produce antisera and hybridomas by immunization between these strains and appropriate fusions; and 5) to perform quality control testing by serology skin grafting and DNA analysis of pedigreed animals in the colony.

该合同的目标是：1）维护 严格的血统 兄弟姐妹的陈设； 2）繁殖和开发新的Engenic Mouse 菌株； 3）在这些之间进行选择性杂交和反向交叉 菌株； 4）通过免疫产生抗血清和杂交瘤 在这些菌株和适当的融合之间； 5）表演 质量控制测试通过血清学皮肤移植和DNA分析 殖民地中的血统动物。