Establishing Automated Cryopreservation System for Biospecimen Storage

建立生物标本储存自动化冷冻保存系统

• 批准号: 10533660
• 负责人: Camila Hochman-Mendez
• 金额: $ 22.08万
• 依托单位: TEXAS HEART INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10533660
• 关键词: Accreditation; Advertising; Agreement; American; Attention; Automation; Back; Basic Science; Biological; Biological Specimen Banks; Biomedical Research; Cardiovascular system; Clinical; Clinical Trials; Collection; Communities; Complex; Computer software; Consumption; Core Facility; Cryopreservation; Data; Development; Disasters; Disease; Emergency Situation; Ensure; Environment; Equipment; Equipment and supply inventories; Exposure to; Foundations; Freezing; Funding; Goals; Grant; Heart; High temperature of physical object; Hour; Human Resources; Infrastructure; Institutes; International; Laboratories; Liquid substance; Maintenance; Manuals; Modernization; Monoclonal Antibody R24; Nitrogen; Outcome; Pathologist; Performance; Play; Preparation; Procedures; Process; Provider; Quality Control; Reaction Time; Reproducibility; Research; Research Personnel; Research Project Grants; Resource Sharing; Retrieval; Role; Sampling; Secure; Services; Societies; Speed; Standardization; System; Technology; Temperature; Texas; Time; Training; Translational Research; United States Food and Drug Administration; base; biobank; cardiovascular health; college; cost; demographics; evidence base; improved; operation; precision medicine; preservation; repository; sample collection; specific biomarkers

Biobanking has been playing an increasingly essential role in biomedical research, growing from simple biospecimen repositories to large complex infrastructure-based systems. With the emergence of omics-based technologies and the ever-increasing demand of biospecimens, there is a recent surge in the number of biorepositories. Consequently, biorepositories are undergoing modernization by implementing state-of-the-art features of sample acquisition, quality control, storage and preservation, and analysis of disease-specific biomarkers, supplemented with well-annotated demographics and clinical/biological data, with the goal of precision medicine outcomes. The Texas Heart Institute (THI)’s College of American Pathologists-accredited and US Food and Drug Administration- and International Society for Biological and Environmental Repositories -compliant Biorepository and Biospecimen Profiling Core Laboratory (THI-BRC) has a large liquid nitrogen (LN2) cryopreservation system for long-term preservation of biospecimens. As a shared resource facility, THI-BRC is providing valuable service, such as sample (mainly cardiovascular) collection, quality-control, processing, storage and preservation, analysis, and inventory maintenance, to internal THI investigators and collaborators throughout the nation, supporting various federal/state/nongovernment-funded basic science and translational research projects and clinical trials. Given the increasing need of large numbers of high-quality samples by our investigators and collaborators, particular attention is needed for sample acquisition, preparation, preservation, retrieval, and inventory management, which can be met by installing an automated cryopreservation workstation. This project will involve installation of the BioStore Cryo III automated cryopreservation system in THI-BRC and its integration into our biorepository current practices, including the development of equipment-specific standard operating procedures (Aim 1), and training THI-BRC personnel in the use and maintenance of this automated cryopreservation system and advertising to the research community the availability of this modernized storage system with increased sample storage capabilities and quality control (Aim 2). Importantly, the proposed system does not require any changes to the current infrastructure. Automated cryopreservation will be implemented to varying degrees at various stages of our biorepository operations to improve sample access, cost, and throughput, offering both quantitative and substantial benefits to the workflow, such as integrated sample traceability, secure storage, increased retrieval speed, and sample preservation. The operation workflow in our biorepository will be standardized and controlled while reducing the time of rack exposure outside the LN2 freezer temperature. Along with mitigating temperature fluctuation of not-intended-to-be-thawed biospecimens, thereby maintaining structural integrity and functional attributes of the biospecimens, the automated cryopreservation system will overcome time-consuming manual processes, improve the efficiency of workflow, and benefit THI’s internal and external investigators’ basic and translational research initiatives on cardiovascular health.

生物样本库在生物医学研究中发挥着越来越重要的作用，从简单的发展到 随着基于组学的出现，生物样本存储库到大型复杂的基于基础设施的系统。 随着技术的进步和生物样本需求的不断增加，最近生物样本的数量激增 经过检查，生物样本库正在通过实施最先进的技术进行现代化改造。 样品采集、质量控制、储存和保存以及疾病特异性分析的特点 生物标志物，辅以详细注释的人口统计数据和临床/生物学数据，目的是 精准医疗结果获得德克萨斯心脏研究所 (THI) 的美国病理学家学院认可。 和美国食品和药物管理局以及国际生物和环境储存库协会 -符合标准的生物样本库和生物样本分析核心实验室（THI-BRC）拥有大型液氮（LN2） 用于长期保存生物样本的冷冻保存系统 作为共享资源设施，THI-BRC 是一个共享资源设施。 提供有价值的服务，例如样本（主要是心血管）采集、质量控制、处理、 存储和保存、分析和库存维护，供内部 THI 研究人员和合作者使用 在全国范围内，支持各种联邦/州/非政府资助的基础科学和转化 鉴于我们对大量高质量样本的需求不断增加。 研究人员和合作者需要特别注意样本采集、准备、保存、 检索和库存管理，这可以通过安装自动化冷冻保存工作站来实现。 该项目将涉及在 THI-BRC 和 THI-BRC 安装 BioStore Cryo III 自动冷冻保存系统 将其整合到我们生物样本库的当前实践中，包括制定设备特定标准 操作程序（目标 1），并培训 THI-BRC 人员使用和维护该自动化设备 冷冻保存系统并向研究界宣传这种现代化存储的可用性 系统具有增强的样品存储能力和质量控制（目标 2）。 不需要对当前基础设施进行任何改变。 我们在生物样本库操作的各个阶段都不同程度地改善样本获取、成本和 吞吐量，为工作流程提供定量和实质性的好处，例如集成样本 我们的操作流程可追溯性、安全存储、提高检索速度和样品保存。 生物样本库将得到标准化和控制，同时减少架子暴露在液氮冷冻柜外的时间 温度同时减轻不打算解冻的生物样本的温度波动，从而 保持生物样本的结构完整性和功能属性，自动冷冻保存 系统将克服耗时的手动流程，提高工作流程的效率，并使 THI 受益 内部和外部研究人员关于心血管健康的基础和转化研究举措。