Regulation of Skeletal progenitor cells in Osteogenesis Imperfecta

成骨不全中骨骼祖细胞的调节

基本信息

批准号：
10528208
负责人：
Brendan Lee
金额：
$ 66.24万
依托单位：
BAYLOR COLLEGE OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-07-13 至 2027-04-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10528208
关键词：
Affect Animal Model Biological Bone Diseases Bone Injury Bone Marrow Bone Regeneration Breathing CCR5 gene Cell Count Cell Proliferation Cell model Cell physiology Cells Characteristics Clinical Clinical Trials Collagen Craniofacial Abnormalities Defect Deformity Disease Environment Extracellular Matrix Face Fracture Gene Expression Profile Genetic Diseases Goals Heterogeneity Human Impaired healing Injury KDR gene Label Ligands Location Maintenance Mastication Methods Modeling Modification Molecular Mus Mutation Natural regeneration Oral Osteoblasts Osteogenesis Imperfecta Patients Periosteal Cell Periosteum Population Procollagen RANTES Regulation Reporter Series Severities Signal Transduction Site Source Surface Tail Technology Testing Transforming Growth Factor beta Transplantation Vascular Endothelial Growth Factors Wild Type Mouse Work base bone bone healing bone repair bone strength cell motility clinically relevant combinatorial cortical bone craniofacial craniofacial bone craniofacial disorder fracture risk improved in vivo insight intravital imaging long bone migration mouse model neutralizing antibody novel osteoprogenitor cell postnatal progenitor receptor restoration selective expression single cell analysis single-cell RNA sequencing skeletal skeletal disorder skeletal stem cell stem stem cell function stem cells success

项目摘要

PROJECT SUMMARY Osteogenesis Imperfecta (OI) is a congenital bone and oral-facial disorder that mainly affects bone with less well characterized alterations in bone healing and repair. Like other skeletal diseases, at least some of the progressive bone and craniofacial defects in OI patients have been attributed to changes in the populations and functions of their stem/progenitor cells. In general, stem cells require a specialized environment for their maintenance and function. Nevertheless, how normal or defective structural components of collagen regulate skeletal stem/progenitor cells (SSPCs) is essentially unknown. Therefore, the goal of this proposal is to define the in vivo characteristics and function of skeletal stem/progenitor cells in OI and to understand how an abnormal OI extracellular matrix alters these stem/progenitor cells in the context of bone regeneration and repair. We previously showed that the Mx1Cre and αSMAGFP combination can selectively label skeletal stem cells in the periosteum and that these Mx1ÈαSMAGFPÈ periosteal cells are long-term repopulating stem cell subsets responsible for lifelong regeneration of periosteal osteoblasts and bone repair. Moreover, our preliminary study revealed that these periosteal SSPCs are Prx1GFP positive and selectively express KDR (VEGFR2). Human primary periosteal cells also express KDR with multi-lineage differentiation potentials. Notably, we found that these KDRÈ periosteal progenitor cells are significantly decreased in OI bones. Hence, we hypothesize that the abnormal OI matrix deregulates the number and function of periosteal and bone marrow SSPCs and the OI- associated molecular changes in stem/progenitor cells are critical for the progressive deformity and delayed or defective healing of bones. By using a series of OI animal models and SSPC reporter mice, in which we can differentially label periosteal and bone marrow SSPC subsets, in combination with intravital imaging and the latest single-cell RNA-sequencing technology, we plan to pursue the following specific aims. In aim 1, we will define the in vivo characteristics and function of periosteal and bone marrow SSPCs in clinically relevant OI mouse models. In aim 2, we will define key OI matrix factors that regulate SSPC function and improve both craniofacial and long bone healing. Upon completion of this work, we will achieve new biological insights into a better understanding of the molecular and cellular mechanisms that differentially regulate SSPCs under OI pathophysiological conditions.

项目概要成骨不全症 (OI) 是一种先天性骨骼和口腔面部疾病，主要影响骨骼，但病情较差与其他骨骼疾病一样，至少有一些骨骼疾病会出现骨愈合和修复的改变。成骨不全患者的进行性骨和颅面缺陷被归因于人口的变化和干细胞/祖细胞的功能一般来说，干细胞需要特殊的环境。然而，胶原蛋白的正常或有缺陷的结构成分如何调节。骨骼干/祖细胞（SSPC）本质上是未知的，因此，该提案的目标是定义。成骨不全症中骨骼干细胞/祖细胞的体内特征和功能，并了解成骨不全症中骨骼干细胞/祖细胞的体内特征和功能异常的 OI 细胞外基质在骨再生过程中改变这些干细胞/祖细胞我们之前表明，Mx1Cre 和 αSMAGFP 组合可以选择性地标记骨骼干。骨膜细胞，这些 Mx1ÈαSMAGFPÈ 骨膜细胞是长期再生干细胞负责骨膜成骨细胞终身再生和骨修复的亚群。研究表明，这些骨膜 SSPC 呈 Prx1GFP 阳性，并选择性表达 KDR (VEGFR2)。原代骨膜细胞也表达具有多谱系分化潜力的 KDR。这些 KDRÈ 骨膜祖细胞在 OI 骨骼中显着减少。异常的 OI 基质会失调骨膜和骨髓 SSPC 的数量和功能以及 OI- 干细胞/祖细胞中相关的分子变化对于进行性畸形和延迟或通过使用一系列 OI 动物模型和 SSPC 报告小鼠，我们可以结合活体成像和借助最新的单细胞 RNA 测序技术，我们计划实现以下具体目标：在目标 1 中，我们将实现以下目标。定义骨膜和骨髓 SSPC 在临床相关 OI 中的体内特征和功能在目标 2 中，我们将定义调节 SSPC 功能并改善两者的关键 OI 矩阵因子。完成这项工作后，我们将对颅面和长骨愈合产生新的生物学见解。更好地了解 OI 下差异调节 SSPC 的分子和细胞机制病理生理条件。