ANGIOGENESIS AND THE ROLE OF XEGR1 IN XENOPUS

爪蟾中的血管生成和 XEGR1 的作用

基本信息

批准号：
2205005
负责人：
MARGARET S SAHA
金额：
$ 10.43万
依托单位：
COLLEGE OF WILLIAM AND MARY
依托单位国家：
美国
项目类别：
财政年份：
1995
资助国家：
美国
起止时间：
1995-06-01 至 1999-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/2205005
关键词：
G protein Xenopus angiogenesis angiogenesis factor antisense nucleic acid cell differentiation early embryonic stage gene expression in situ hybridization mixed tissue /cell culture protein tyrosine kinase receptor coupling receptor expression scanning electron microscopy vascular endothelium

项目摘要

DESCRIPTION (Adapted from the applicant's abstract): To sustain growth and differentiation, embryos must create a functional circulatory system during early development. Little is known about the tissue interactions that may be required to induce the formation of angioblasts (vessel endothelial precursors). The investigator proposes first to examine the spatial and temporal expression pattern for cells that express an early vascular endothelium-specific marker, xEGR1 (an Endothelium G-Protein coupled Receptor), and a putative receptor tyrosine kinase homolog of vascular-specific mouse genes flk-1 and flt. She also proposes to determine the region of the frog embryo that gives rise to angioblasts by creating a precise fate map of cells that later express xEGR1, and further to examine the appearance of developing vacular tissue with scanning electron microscopy. Second, she proposes to examine tissue interactions that may be important in angioblast induction by using tissue co-cultures and treatment of undetermined tissues with various known mesoderm inducers or agents known to disrupt mesoderm specification. Finally, she proposes to investigate whether xEGR1 has a direct role in angiogenesis/vasculogenesis by forcing ectopic expression of xEGR1 and by treating cells with agonists and antagonists of xEGR1 protein.

描述（根据申请人的摘要改编）：维持增长和差异化，胚胎必须创建功能性循环系统在早期发展期间。关于组织相互作用知之甚少诱导血管细胞的形成可能需要内皮前体）。研究人员首先建议及早表达的细胞的空间和时间表达模式血管内皮特异性标记，XEGR1（内皮G蛋白耦合受体），以及推定的受体酪氨酸激酶同源物血管特异性小鼠基因FLK-1和FLT。她还建议确定青蛙胚胎的区域，产生血管生成细胞通过创建一个细胞的精确命运图，后来表达Xegr1，然后进一步检查与扫描电子显微镜。其次，她建议检查组织通过使用在血管生成诱导中可能很重要的相互作用组织共培养和处理不确定的组织已知的中胚层诱导剂或已知破坏中胚层的药物规格。最后，她建议调查Xegr1是否有通过强迫异位来在血管生成/血管生成中的直接作用 XEGR1的表达和通过激动剂和拮抗剂处理细胞 XEGR1蛋白。

项目成果

期刊论文数量（6）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Tissue-specific developmental expression of OAX, a Xenopus repetitive element.

OAX（非洲爪蟾重复元件）的组织特异性发育表达。

DOI：
10.1016/s0925-4773(00)00307-5
发表时间：
2000
期刊：
Mechanisms of development
影响因子：
2.6
作者：
Whitford,KL;Oakes,JA;Scholnick,J;Saha,MS
通讯作者：
Saha,MS

Short upstream region drives dynamic expression of hypoxia-inducible factor 1alpha during Xenopus development.

短上游区域驱动爪蟾发育过程中缺氧诱导因子 1α 的动态表达。

DOI：
10.1002/dvdy.20049
发表时间：
2004
期刊：
Developmental dynamics : an official publication of the American Association of Anatomists.
影响因子：
0
作者：
Sipe,ConorW;Gruber,ErikaJ;Saha,MargaretS
通讯作者：
Saha,MargaretS

Elucidating the origins of the vascular system: a fate map of the vascular endothelial and red blood cell lineages in Xenopus laevis.

阐明血管系统的起源：非洲爪蟾血管内皮细胞和红细胞谱系的命运图。