CORE--ADVANCED TECHNOLOGY LABORATORIES

核心--先进技术实验室

• 批准号: 5205150
• 负责人: WILLIAM G POWDERLY
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/5205150
• 关键词: AIDS; AIDS therapy; HIV infections; antiAIDS agent; antibody neutralization test; biomarker; biomedical facility; clinical research; cooperative study; cytokine; drug metabolism; human immunodeficiency virus 1; human subject; human therapy evaluation; immunity; immunology; neutralizing antibody; pathologic process; pharmacokinetics; pharmacology; polymerase chain reaction; serology /serodiagnosis; virus antigen

The Core Retrovirus Laboratory of the Washington University ACTU is currently a state-of-the-art facility for performing HIV diagnostic testing. The scope of operations includes lymphocyte co-cultivation for HIV, ELISA testing for p24 antigen, ELISA and western blot for HIV antibodies, and PCR for HIV DNA. Techniques for HIV antiviral susceptibility testing and RNA PCR are also available. Competence in p24 antigen detection and HIV co-cultivation have been demonstrated in the ACTG quality control program. Electronic communications with Frontier Science Technology Research Foundation (FSTRF) and the Washington University clinical facility are fully operational and are used for electronic transport of data. The Retrovirus Laboratory Management Program (RLMP) is fully operational and is used for specimen tracking and freezer inventories. Use of semi-automated equipment and instrument interfaces to the computer system allow high specimen throughput, and along with the RLMP have made possible prompt and efficient responses to all requests for p24 batch testing, with electronic export of complete data in the proper format. These systems could be adapted to allow the laboratory to receive and process specimens from other ACTUs, with considerable economies resulting from increased testing volume. Performance of 200 p24 antigen assays and 40-50 HIV cultures per week is feasible, and could be accomplished with considerably less than proportional increase in laboratory personnel. In addition to the laboratory's strong record in ACTU-related activities, a high level of expertise exists in other areas of HIV virology. The laboratory currently is capable of performing PCR for HIV DNA and RNA, quantitative plasma and cell cultures, antiviral susceptibility testing and monocyte cultures. The laboratory is involved in several research projects in which these techniques are used and the technical experience can be translated for use with ACTG protocols when the need arises. The laboratory directors are experienced virologists and both are involved in ACTG activities at the committee level. All other laboratory personnel are proficient in both technical procedures and in operating the relevant electronic information systems.

华盛顿大学Actu的核心逆转录病毒实验室是 目前是执行HIV诊断的最先进的设施 测试。 操作范围包括淋巴细胞共培养 HIV，ELISA测试p24抗原，ELISA和Western blot for HIV 抗体和HIV DNA的PCR。 艾滋病毒抗病毒技术 还提供了敏感性测试和RNA PCR。 P24的能力 ACTG已证明了抗原检测和HIV共培养 质量控制计划。 与边境科学的电子通信 技术研究基金会（FSTRF）和华盛顿大学 临床设施完全运行，用于电子 数据传输。 逆转录病毒实验室管理计划（RLMP）是 完全运行，用于标本跟踪和冰柜 库存。 使用半自动化设备和仪器接口到 计算机系统允许高标本吞吐量，以及RLMP 已经使对P24的所有请求做出了迅速，有效的响应 批处理测试，并以适当的电子导出完整数据出口 格式。 可以对这些系统进行调整以允许实验室接收 和来自其他Actus的过程标本，具有相当大的经济体 由于测试量增加而产生。 200 p24抗原的性能 测定和每周40-50种艾滋病毒培养是可行的，可能是 完成的成绩远小于比例增加 实验室人员。 除了实验室在与ACTU有关的活动中的良好记录外， HIV病毒学的其他领域存在高水平的专业知识。 这 当前实验室能够对HIV DNA和RNA进行PCR， 定量等离子体和细胞培养物，抗病毒易感性测试和 单核细胞培养。 该实验室参与了几个研究项目 在其中使用这些技术，并且技术经验可以是 需要在需要时与ACTG协议一起使用。 实验室主任是经验丰富的病毒学家，都参与其中 在委员会一级的ACTG活动中。 所有其他实验室人员 精通技术程序和操作相关的 电子信息系统。