CYTOKINES AND BONE TURNOVER IN THYROID DISORDERS

甲状腺疾病中的细胞因子和骨转换

基本信息

批准号：
2291642
负责人：
PAULA H STERN
金额：
$ 2.47万
依托单位：
NORTHWESTERN UNIVERSITY AT CHICAGO
依托单位国家：
美国
项目类别：
财政年份：
1993
资助国家：
美国
起止时间：
1993-02-01 至 1995-01-31
项目状态：
已结题

项目摘要

Thyroid hormone excess can cause bone loss and may be a potential risk factor for secondary osteoporosis for large numbers of the population. Menopause may aggravate these effects of thyroid hormones. Although the relationship between thyroid hormones and bone metabolism has been well documented, the mechanism of action of these hormones on bone has not yet been fully elucidated. Since there is a well-defined relationship between the immune system and thyroid hormones, suggesting that thyroid hormones stimulate the production of cytokines by immune cells. Thyroid hormones might stimulate the synthesis of these factors in other tissues, as well, resulting in elevated serum levels of the cytokines. A number of cytokines play important roles in the regulation of bone metabolism. Therefore, we postulate that certain cytokines participate in the mediation of thyroid hormone effects on bone. In the proposed study, we plan to investigate the changes in the circulating levels of cytokines involved in bone metabolism, including interleukin-1alpha (IL-1alpha), interleukin-1beta (IL-1beta), interleukin-6 (IL-6) and insulin-like growth factor I (IFG-I). A total of 90 subjects will be examined including pre- and postmenopausal healthy women and men, as well as patients with hyper- and hypothyroidism before and after treatment. Bone biochemical parameters, including serum osteocalcin and urinary hydroxyproline excretion, bone mineral content, using single and dual photon absorptiometry, and IL-1alpha and beta secretion by monocyte cultures isolated from patients' blood will also be measured before and after treatment. Serum bone resorbing activity (SBRA) of sera showing high and low cytokine levels will be determined in fetal rat limb bone cultures. Data will be analyzed in order to determine whether there are correlations between the effects of thyroid hormones on the production of cytokines and bone metabolism.

甲状腺激素过量会导致骨质流失，可能是潜在的风险大量人群的继发性骨质疏松症因子。更年期可能会加重甲状腺激素的这些作用。虽然甲状腺激素与骨代谢之间的关系一直很好记录到，这些激素在骨骼上的作用机理尚未被完全阐明。由于有一个明确的关系在免疫系统和甲状腺激素之间，表明甲状腺激素刺激免疫细胞的细胞因子产生。甲状腺激素可能会刺激其他组织中这些因素的合成，同样，导致细胞因子的血清水平升高。一个数字细胞因子的作用在调节骨代谢中起着重要作用。因此，我们假设某些细胞因子参与甲状腺激素对骨骼的影响。在拟议的研究中，我们计划研究细胞因子循环水平的变化参与骨代谢，包括白介素-1alpha（IL-1Alpha），白介素1beta（IL-1Beta），白介素6（IL-6）和类似胰岛素样生长因子I（IFG-I）。总共将检查90名受试者包括绝经前和后健康的男女治疗前后患有甲状腺功能亢进和甲状腺功能低下的患者。骨生化参数，包括血清骨钙素和尿羟基的排泄，骨矿物质含量，使用单一和双重单核细胞的光子吸收率和IL-1Alpha和β分泌从患者血液中分离出的培养物也将在和治疗后。血清血清血清血清的血清表现高和低细胞因子水平将在胎儿大鼠肢体中确定文化。将分析数据以确定是否存在甲状腺激素对生产的影响之间的相关性细胞因子和骨代谢。