SURFACE ANTIGENS OF ENDOSYMBIOTIC DIATOMS

内共生硅藻的表面抗原

• 批准号: 3756022
• 负责人: JOHN J LEE
• 金额: --
• 依托单位: CITY COLLEGE OF NEW YORK
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3756022
• 关键词: algae; alternatives to animals in research; antibody formation; antibody specificity; biochemical evolution; chemical binding; electrophoresis; host organism interaction; iodine; laboratory rat; molecular shape; peptide chemical synthesis; radioimmunoassay; radionuclides; species difference; surface antigens; symbiosis; systematic biology; western blottings

"Living sands are major primary producers and sinks of nutrients and carbonate in oligotrophic seas. Although all living sands contain one of a wide diversity of algal types, the diatom-bearing larger foraminifera are a unique form of the symbiotic phenomenon. So far, 19 different, and taxonomically widely separated, diatom species have been isolated from hosts, although usually only one species of diatom has been observed at a time in a single host. The diatoms which form endosymbioses are extremely rare in the habitat where the forams are found. Foraminifera eat mainly diatoms. Although symbionts are passed from generation to generation by asexual reproduction, the flagellated gametes released into the water column are too small to contain symbionts. The chain is broken unless the zygotes or young agamonts find new symbionts. How do they recognize them? Nothing is known about initial interactions. Some species in the same genus serve as food while other species become endosymbionts. Western blotting will be used to screen cross-reactive surface antigens and to affinity purify antibodies to specific antigens by eluting bound antibodies of interest. Later dot blot immunoassays will be used. After absorption of the antisera, blocking experiments will be performed. Fine structural studies will aid understanding the process. Human activities in most of their range (e.g. Persian Gulf) threaten their habitats and survival. We know little about their resistance or what their loss will mean to the global C02 balance.

“活沙是营养成分的主要主要生产者和水槽， 碳酸盐在贫营养海洋中。 虽然所有活沙子都包含一个 多种藻类类型的多样性，含硅藻的较大 有孔虫是共生现象的独特形式。 到目前为止，19 不同的和分类广泛分离的硅藻已经存在 从宿主分离出来，尽管通常只有一种硅藻 一次在一个主机中观察到。 形成的硅藻 内共生植物在山孔是的栖息地极为罕见 成立。 有孔虫主要吃硅藻。 虽然通过了共生体 通过无性繁殖一代，鞭毛 释放到水柱的配子太小而无法包含 共生体。 除非Zygotes或Young Agamonts发现 新的共生体。 他们如何认识他们？ 什么都不知道 初始互动。 同一属中的某些物种作为食物，而 其他物种成为内共生。 蛋白质印迹将用于筛选交叉反应性表面抗原 并通过洗脱结合来纯化对特定抗原的抗体 感兴趣的抗体。 稍后将使用DOT印迹免疫测定。 后 抗血清的吸收，将进行阻塞实验。 美好的 结构研究将有助于理解这一过程。 人类活动的大部分范围（例如波斯湾）威胁 他们的栖息地和生存。 我们对他们的抵抗力一无所知或 他们的损失对全球C02余额意味着什么。