THE ROLE OF LECTINS IN SNAIL-TREMATODE ASSOCIATIONS

凝集素在蜗牛-吸虫协会中的作用

• 批准号: 3755957
• 负责人: ERIC S LOKER
• 金额: --
• 依托单位: UNIVERSITY OF NEW MEXICO
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3755957
• 关键词: Gastropoda; Mollusca; Trematoda; affinity chromatography; disease /disorder model; electrophoresis; hamsters; hemolymph; host organism interaction; immune system; laboratory mouse; laboratory rabbit; larva; lectin; Gastropoda; Mollusca; Trematoda; affinity chromatography; disease /disorder model; electrophoresis; hamsters; hemolymph; host organism interaction; immune system; laboratory mouse; laboratory rabbit; larva; lectin

Several aspects of the immunobiology of invertebrates remain poorly known relative to our detailed understanding of the vertebrate immune response. Because many invertebrates serve as intermediate hosts or vectors of parasites of great medical or veterinary significance, it is imperative that we better understand how their immune systems recognize and respond to infection by parasites. Consequently, the long term goal of the proposed research is to elucidate the fundamental immunological mechanisms underlying susceptibility and resistance of freshwater snails to infection with larval digenetic trematodes. Using as a model system the snail Biomphalaria glabrata and the trematode Echinostoma paraensei, snail hemolymph lectins will be investigated as mediators of "non-self" recognition in interactions with trematode larvae. Prior work with other molluscs and with this particular model system provide strong justification for this effort. A comprehensive series of experiments is proposed to explore thoroughly the functional relevance of lectins to this host- parasite system. Using a combination of electrophoretic techniques and specific antibody probes, both circulating lectins and hemocyte-associated lectins (hemocytes are molluscan immune cells) will be studied to determine if lectin composition changes following exposure to trematode infection. Also, lectins produced by snails known to be susceptible or resistant to E. paraensei infection will be carefully examined for differences that may relate to immune competence. Lectins purified by affinity chromatography will be tested for their ability to mediate hemocyte attachment to (and killing of) trematode larvae. Experiments will also be undertaken to determine if hemocytes are responsible for synthesizing lectin molecules, and if secretory products released by trematode larvae can interfere with the production of, or anti-parasite functions of, lectins. The proposed work will identify snail-produced molecules with anti-parasite functions and will set the stage for future attempts to clone the corresponding genes. Potentially, such efforts will culminate in the development of innovative new approaches to trematode control.

无脊椎动物免疫生物学的几个方面仍然鲜为人知 相对于我们对脊椎动物免疫反应的详细理解。 因为许多无脊椎动物是中间主机或向量 具有出色的医学或兽医意义的寄生虫，这是必须的 我们更好地了解他们的免疫系统如何认识和回应 寄生虫感染。 因此，提议的长期目标 研究是为了阐明基本的免疫机制 淡水蜗牛对感染的潜在敏感性和阻力 带有幼虫挖掘障碍。 用作模型系统蜗牛 Biomphalaria glabrata和Trematode echinostoma paraensei，蜗牛 血淋巴凝集素将作为“非自身”的介体进行研究 在与Trematode幼虫相互作用中的识别。 与其他人的事先工作 软体动物和此特定模型系统提供了强有力的理由 为了这项努力。 提出了一系列全面的实验 彻底探索凝集素与该宿主的功能相关性 - 寄生虫系统。 结合电泳技术和 特定的抗体探针，循环凝集素和与血细胞相关的探针 将研究凝集素（血细胞是软体动物免疫细胞）以确定 如果暴露于Trematode感染后凝集素组成发生了变化。 同样，蜗牛产生的凝集素已知易感或抗E。 Paraensei感染将经过仔细检查是否可能 与免疫能力有关。 通过亲和色谱纯化的凝集素 将测试其介导血细胞附着的能力（和 杀死）trematode幼虫。 实验也将进行 确定血细胞是否负责合成凝集素分子， 如果Trematode幼虫发布的分泌产品可能会干扰 凝集素的产生或抗寄生虫功能。 提议 工作将识别具有抗寄生虫功能的蜗牛产生的分子 并将为未来的尝试克隆相应的尝试奠定基础 基因。 可能，这种努力将在发展中 创新的新方法来控制Trematode。